Geum, Na Gyeong;Yu, Ju-Hyeong;Yeo, Joo Ho;Choi, Min Yeong;Lee, Jae Won;Beak, Jueng Kyu;Jeong, Jin Boo published 《Immunostimulatory activity and anti-obesity activity of Hibiscus manihot leaves in mouse macrophages, RAW264.7 cells and mouse adipocytes, 3T3-L1 cells》 in 2021. The article was appeared in 《Journal of Functional Foods》. They have made some progress in their research.SDS of cas: 243984-11-4 The article mentions the following:
In this study, we investigated whether Hibiscus manihot leaves (HML) exhibits immune-enhancing activity and anti-obesity in RAW264.7 and 3T3-L1 cells. HML increased the production of inflammatory mols., cell viability and phagocytosis in RAW264.7 cells. TLR2 and TLR4 blocked HML-mediated production of inflammatory mols. in RAW264.7 cells. In addition, the inhibition of MAPK signaling pathway reduced HML-mediated production of inflammatory mols. and the activation of MAPK signaling pathway by HML suppressed the inhibition of TLR2/4. HML reduced the lipid accumulation and TG contents in 3T3-L1 cells. HML inhibited the protein expressions such as CEBPα, PPARγ, perilipin-1, adiponectin and FABP4 related to the lipid accumulation of the mature adipocytes. In addition, HML reduced CEBPα and PPARγ during the differentiation process from preadipocytes to mature adipocytes. The experimental procedure involved many compounds, such as (R)-Ethyl 6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-enecarboxylate (cas: 243984-11-4) .
(R)-Ethyl 6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-enecarboxylate(cas:243984-11-4) is a toll-like receptor 4 (TLR4) signaling inhibitor.SDS of cas: 243984-11-4 And it can inhibits LPS-induced cytokine production in vitro (IC50 values are 1.3, 1.3 and 3.2 nM for IL-6, TNFα and NO production).
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