Zdovc, Blaz;Jaklin, Matej;Hribar-Lee, Barbara;Luksic, Miha published 《Influence of Low Molecular Weight Salts on the Viscosity of Aqueous-Buffer Bovine Serum Albumin Solutions》. The research results were published in《Molecules》 in 2022.Application of 7791-11-9 The article conveys some information:
Pharmaceutical design of protein formulations aims at maximum efficiency (protein concentration) and min. viscosity. Therefore, it is important to know the nature of protein-protein interactions and their influence on viscosity. In this work, we investigated the dependence of the viscosity of BSA in an aqueous 20 mM acetate buffer at pH = 4.3 on protein concentration and on temperature (5-45°C). The viscosity of the solution increased with protein concentration and was 230% higher than the viscosity of the protein-free formulation at 160 mg/mL. The viscosity decreased by almost 60% in the temperature range from 5 to 45°C. The agreement of the modified Arrhenius theory with experiment was quant., whereas a hard-sphere model provided only a qual. description of the exptl. results. We also investigated the viscosity of a 100 mg/mL BSA solution as a function of the concentration of added low mol. weight salts (LiCl, NaCl, KCl, RbCl, CsCl, NaBr, NaI) in the range of salt concentrations up to 1.75 mol/L. In addition, the particle size and zeta potential of BSA-salt mixtures were determined for solutions containing 0.5 mol/L salt. The trends with respect to the different anions followed a direct Hofmeister series (Cl– > Br– > I–), whereas for cations in the case of viscosity the indirect Hofmeister series was observed (Li+ > Na+ > K+ > Rb+ > Cs+), but the values of particle sizes and zeta potential did not show cation-specific effects. Since the protein is pos. charged at pH = 4.3, anions are more attracted to the protein surface and shield its charge, while the interaction with cations is less pronounced. We hypothesize that salt surface charge shielding reduces protein colloidal stability and promotes protein aggregate formation. To complete the study, the researchers used Rubidiumchloride (cas: 7791-11-9) .
Rubidium chloride(cas: 7791-11-9) is the mostly used rubidium compound, and finds use in various fields ranging from electrochemistry to molecular biology.Application of 7791-11-9 It is an efficient non-invasive biomarker; increases dopamine and norepinephrine levels, and useful for anergic and apathetic depressives.
Reference:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics