Month: September 2022

COA of Formula: C10H13ClO3《Enzymatic Process for N-Substituted (3S)- and (3R)-3-Hydroxypyrrolidin-2-ones》 was published in 2015. The authors were Singh, Amarjit;Falabella, James;LaPorte, Thomas L.;Goswami, Animesh, and the article was included in《Organic Process Research & Development》. The author mentioned the following in the article:

Lipase PS 30 (immobilized on polypropylene) from Pseudomonas cepacia was used for enantioselective esterification of (RS)-1-(2-fluoro-4-iodophenyl)-3-hydroxypyrrolidin-2-one by using succinic anhydride and 2-methyltetrahydrofuran at 4 °C. The isolation of desired alc. avoided use of column chromatog., a simple solvent extraction of undesired (R)-4-((1-(2-fluoro-4-iodophenyl)-2-oxopyrrolidin-3-yl)oxy)-4-oxobutanoic acid into 5% potassium bicarbonate solution separated pure desired (S)-1-(2-fluoro-4-iodophenyl)-3-hydroxypyrrolidin-2-one into the 2-methyltetrahydrofuran solution The reaction conditions were optimized, and (S)-1-(2-fluoro-4-iodophenyl)-3-hydroxypyrrolidin-2-one was prepared in high enantiomeric excess >99% and yield ∼40% (theor. possible yield 50%). Novozym 435 (Candida antarctica lipase B) was found to be a suitable biocatalyst for the resolution of (RS)-1-(6-bromo-2-methylpyridin-3-yl)-2-oxopyrrolidin-3-yl acetate to form the undesired S-acetate and the desired R-alc. The optimized reaction conditions gave (R)-1-(6-bromo-2-methylpyridin-3-yl)-3-hydroxypyrrolidin-2-one in ∼37% isolated yield (maximum possible yield 50%) and high enantiomeric excess (ee >99.4%). The enzymic resolution of (RS)-1-(6-bromo-2-methylpyridin-3-yl)-2-oxopyrrolidin-3-yl acetate followed by chromatog. was successfully implemented to deliver material for two successive (4.1 kg, ee >99.4% and 5.5 kg, ee >99.5%) campaigns. The undesired S-alc. was recycled back to the desired R-alc. using a Mitsunobu inversion of stereochem. in gram scale. An increase in the chain length from acetate to hexanoate improved the selectivity and subsequent optimization decreased the enzyme loading and enhanced the substrate input. Separation of the desired (R)-1-(6-bromo-2-methylpyridin-3-yl)-3-hydroxypyrrolidin-2-one from (S)-1-(6-bromo-2-methylpyrrolidin-3-yl)-2-oxopyrrolidin-3-yl hexanoate was achieved using a solvent extraction The process for the preparation of (S)-1-(2-fluoro-4-iodophenyl)-3-hydroxypyrrolidin-2-one and (R)-1-(6-bromo-2-methylpyridin-3-yl)-3-hydroxypyrrolidin-2-one is scalable, economical, and highly efficient and avoids chromatog. To complete the study, the researchers used (1S)-4,7,7-Trimethyl-3-oxo-2-oxabicyclo[2.2.1]heptane-1-carbonyl chloride(Chunks or pellets) (cas: 39637-74-6) .

(1S)-4,7,7-Trimethyl-3-oxo-2-oxabicyclo[2.2.1]heptane-1-carbonyl chloride(Chunks or pellets) (cas: 39637-74-6 COA of Formula: C10H13ClO3) is used as a resolving agent for alcohols as the diastereomeric esters by crystallization or chromatography and as a chiral derivatization reagent for determination of enantiomeric excess of alcohols and amines.

Reference:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Wagner, Stefan;Santner, Jakob;Irrgeher, Johanna;Puschenreiter, Markus;Happel, Steffen;Prohaska, Thomas published 《Selective Diffusive Gradients in Thin Films (DGT) for the Simultaneous Assessment of Labile Sr and Pb Concentrations and Isotope Ratios in Soils》 in 2022. The article was appeared in 《Analytical Chemistry (Washington, DC, United States)》. They have made some progress in their research.Application In Synthesis of Rubidiumchloride The article mentions the following:

A method using diffusive gradients in thin films (DGT) for the accurate quantification of trace-level (μg L^-1) Sr and Pb concentrations and isotope ratios [δ_SRM 987(⁸⁷Sr/⁸⁶Sr) and δ_SRM 981(²⁰⁷Pb/²⁰⁶Pb)] in labile, bioavailable element fractions in soils is reported. The method is based on a novel poly(tetrafluoroethylene) (PTFE) membrane binding layer with combined di(2-ethyl-hexyl)phosphoric acid (HDEHP) and 4,4′(5′)-bis-t-butylcyclohexano-18-crown-6 (crown-ether) functionality with high selectivity for Sr and Pb (TK100 membrane). Laboratory evaluation of the TK100 DGT showed linear uptake of Sr over time (2-24 h) up to very high Sr mass loadings on TK100 membranes (288μg cm^-2) and effective performance in the range of pH (3.9-8.2), ionic strength (0.001-0.1 mol L^-1), and cation competition (50-160 mg L^-1 Ca in a synthetic soil solution matrix) of environmental interest. Selective three-step elution of TK100 membranes using hydrochloric acid allowed us to obtain purified Sr and Pb fractions with adequate (≥75%) recovery and quant. (≥96%) matrix reduction Neither DGT-based sampling itself nor selective elution or mass loading effects caused significant isotopic fractionation. Application of TK100 DGT in natural soils and comparison with conventional approaches of bioavailability assessment demonstrated the method’s unique capability to obtain information on Sr and Pb resupply dynamics and isotopic variations with low combined uncertainty within a single sampling step. To complete the study, the researchers used Rubidiumchloride (cas: 7791-11-9) .

Rubidium chloride(cas: 7791-11-9) is the mostly used rubidium compound, and finds use in various fields ranging from electrochemistry to molecular biology.Application In Synthesis of Rubidiumchloride It is an efficient non-invasive biomarker; increases dopamine and norepinephrine levels, and useful for anergic and apathetic depressives.

Reference:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Rong, Bohan;Wu, Qiong;Reiter, Russel J.;Sun, Chao published 《The Mechanism of Oral Melatonin Ameliorates Intestinal and Adipose Lipid Dysmetabolism Through Reducing Escherichia Coli-Derived Lipopolysaccharide》. The research results were published in《Cellular and Molecular Gastroenterology and Hepatology》 in 2021.Electric Literature of C15H17ClFNO4S The article conveys some information:

Gut microbiota have been reported to be sensitive to circadian rhythms and host lipometabolism, resp. Although melatonin-mediated beneficial efforts on many physiol. sites have been revealed, the regulatory actions of oral melatonin on the communication between gut microbiota and host are still not clear. Angiopoietin-like 4 (ANGPTL4) has been shown to be strongly responsible for the regulation of systemic lipid metabolism Herein, we identified that oral melatonin improved lipid dysmetabolism in ileum and epididymal white adipose tissue (eWAT) via gut microbiota and ileac ANGPTL4. Analyses of jet-lag (JL) mice, JL mice with oral melatonin administration (JL+MT), and the control for mRNA and protein expression regarding lipid uptake and accumulation in ileum and eWAT were made. Gut microbiome sequencing and exptl. validation of target strains were included. Functional anal. of key factors/pathways in the various rodent models, including the depletion of gut microbiota, mono-colonization of Escherichia coli, and other genetic intervention was made. Analyses of transcriptional regulation and effects of melatonin on E coli-derived lipopolysaccharide (LPS) in vitro were made. JL mice have a higher level of ileal lipid uptake, fat accumulation in eWAT, and lower level of circulating ANGPTL4 in comparison with the control mice. JL mice also showed a significantly higher abundance of E coli and LPS than the control mice. Conversely, oral melatonin supplementation remarkably reversed these phenotypes. The test of depletion of gut microbiota further demonstrated that oral melatonin-mediated improvements on lipometabolism in JL mice were dependent on the presence of gut microbiota. By mono-colonization of E coli, LPS has been determined to trigger these changes similar to JL. Furthermore, we found that LPS served as a pivotal link that contributed to activating toll-like receptor 4 (TLR4)/signal transducer and activator of transcription 3 (STAT3_/REV-ERBα) signaling to up-regulate nuclear factor interleukin-3-regulated protein (NFIL3) expression, resulting in increased lipid uptake in ileum. In MODE-K cells, the activation of NFIL3 has further been shown to inhibit ANGPTL4 transcription, which is closely associated with lipid uptake and transport in peripheral tissues. Finally, we confirmed that melatonin inhibited LPS via repressing the expression of LpxC in E coli. Overall, oral melatonin decreased the quantity of E coli-generated LPS, which alleviated NFIL3-induced transcriptional inhibition of ANGPTL4 through TLR4/IL-22/STAT3 signaling in ileum, thereby resulting in the amelioration of ileal lipid intake and lower fat accumulation in eWAT. These results address a novel regulation of oral melatonin originating from gut microbiota to host distal tissues, suggesting that microbe-generated metabolites are potential therapies for melatonin-mediated improvement of circadian rhythm disruption and related metabolic syndrome. The experimental procedure involved many compounds, such as (R)-Ethyl 6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-enecarboxylate (cas: 243984-11-4) .

(R)-Ethyl 6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-enecarboxylate(cas:243984-11-4) is a toll-like receptor 4 (TLR4) signaling inhibitor.Electric Literature of C15H17ClFNO4S And it can inhibits LPS-induced cytokine production in vitro (IC50 values are 1.3, 1.3 and 3.2 nM for IL-6, TNFα and NO production).

Reference:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Safety of (R)-Ethyl 6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-enecarboxylate《Atorvastatin upregulates microRNA-186 and inhibits the TLR4-mediated MAPKs/NF-κB pathway to relieve steroid-induced avascular necrosis of the femoral head》 was published in 2021. The authors were Zhang, Yusong;Ma, Limin;Lu, Erhai;Huang, Wenhua, and the article was included in《Frontiers in Pharmacology》. The author mentioned the following in the article:

Steroid-induced avascular necrosis of the femoral head (SANFH) is caused by the death of active components of the femoral head owing to hormone overdoses. The use of lipid-lowering drugs to prevent SANFH in animals inspired us to identify the mechanisms involving Atorvastatin (Ato) in SANFH. However, it is still not well understood how and to what extent Ato affects SANFH. This study aimed to figure out the efficacy of Ato in SANFH and the underlying mol. mechanisms. After establishment of the SANFH model, histol. evaluation, lipid metabolism, inflammatory cytokines, oxidative stress, apoptosis, and autophagy of the femoral head were evaluated. The differentially expressed microRNAs (miRs) after Ato treatment were screened out using microarray anal. The downstream gene and pathway of miR-186 were predicted and their involvement in SANFH rats was analyzed. OB-6 cells were selected to simulate SANFH in vitro. Cell viability, cell damage, inflammation responses, apoptosis, and autophagy were assessed. Ato alleviated SANFH, inhibited apoptosis, and promoted autophagy. miR-186 was significantly upregulated after Ato treatment. miR-186 targeted TLR4 and inactivated the MAPKs/NF-κB pathway. Inhibition of miR-186 reversed the protection of Ato on SANFH rats, while inhibition of TLR4 restored the protective effect of Ato. Ato reduced apoptosis and promoted autophagy of OB-6 cells by upregulating miR- 186 and inhibiting the TLR4/MAPKs/NF-κB pathway. In conclusion, Ato reduced apoptosis and promoted autophagy, thus alleviating SANFH via miR-186 and the TLR4-mediated MAPKs/NF-κB pathway. The experimental procedure involved many compounds, such as (R)-Ethyl 6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-enecarboxylate (cas: 243984-11-4) .

(R)-Ethyl 6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-enecarboxylate(cas:243984-11-4) can reduces lesion volume in a mouse model of cerebral cavernous malformations (CCMs).Safety of (R)-Ethyl 6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-enecarboxylate Also attenuates increased cytokine levels in a mouse sepsis model, when given in combination with ceftazidime. Cell permeable.

Reference:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Will, Jonas M.;Behrens, Arne;Macke, Marcel;Quarles, C. Derrick Jr.;Karst, Uwe published 《Automated chiral analysis of amino acids based on chiral derivatization and trapped ion mobility-mass spectrometry》. The research results were published in《Analytical Chemistry (Washington, DC, United States)》 in 2021.Related Products of 39637-74-6 The article conveys some information:

A fast and fully automated method for chiral anal. has been developed by combining a chiral derivatization approach with high-resolution trapped ion mobility separation Although the presented approach can be potentially applied to diverse types of chiral compounds, several benchmark amino acids were used as model compounds, focusing on the smallest amino acid alanine. An autosampler with an integrated chromatog. system was used for inline chiral derivatization with (S)-naproxen chloride and subsequent presepn. Afterwards, derivatized amino acids were directly introduced into the electrospray interface of a trapped ion mobility-mass spectrometer for rapid diastereomer separation in the gas phase. This unique combination of presepn. and trapped ion mobility spectrometry separation in the neg. ion mode enabled rapid chiral anal. within 3 min per sample. Furthermore, the diastereomer separation proved to be independent of alkali salts or other metal ions, offering robustness with regard to samples containing high amounts of salts. Highly sensitive detection of amino acid diastereomers was possible down to the lower nanomolar concentration range, and enantiomeric ratios could be readily determined from the recorded mobilograms with excellent reproducibility and precision. To demonstrate the general applicability of our method, alanine and other amino acids were analyzed from soy sauces and seasonings, which revealed extraordinarily high D-Ala contents of up to 99% in all samples. To complete the study, the researchers used (1S)-4,7,7-Trimethyl-3-oxo-2-oxabicyclo[2.2.1]heptane-1-carbonyl chloride(Chunks or pellets) (cas: 39637-74-6) .

(1S)-4,7,7-Trimethyl-3-oxo-2-oxabicyclo[2.2.1]heptane-1-carbonyl chloride(Chunks or pellets) (cas: 39637-74-6 Related Products of 39637-74-6) is used as a resolving agent for alcohols as the diastereomeric esters by crystallization or chromatography and as a chiral derivatization reagent for determination of enantiomeric excess of alcohols and amines.

Reference:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Liu, Shaoguang;Zhang, Shaotong;Sun, Yulong;Zhou, Wence published 《Transcriptomics Changes in the Peritoneum of Mice with Lipopolysaccharide-Induced Peritonitis》. The research results were published in《International Journal of Molecular Sciences》 in 2021.Safety of (R)-Ethyl 6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-enecarboxylate The article conveys some information:

Peritonitis caused by LPS is a severe clin. challenge, which causes organ damage and death. However, the mechanism of LPS-induced peritonitis has not been fully revealed yet. Here, we investigated the transcriptome profile of the peritoneal tissue of LPS-induced peritonitis in mice. A model of LPS-induced peritonitis in mice was established (LPS 10 mg/kg, i.p.), and the influence of TAK 242 (TLR4 inhibitor) on the level of inflammatory cytokines in mouse peritoneal lavage fluid was investigated by using an ELISA test. Next, the peritoneal tissues of the three groups of mice (Control, LPS, and LPS+TAK 242) (n = 6) were isolated and subjected to RNA-seq, followed by a series of bioinformatics analyses, including differentially expressed genes (DEGs), enrichment pathway, protein-protein interaction, and transcription factor pathway. Then, qPCR verified-hub genes that may interact with TAK 242 were obtained. Subsequently, the three-dimensional structure of hub proteins was obtained by using homol. modeling and mol. dynamics optimization (300 ns). Finally, the virtual docking between TAK 242 and hub proteins was analyzed. Our results showed that TAK 242 significantly inhibited the production of inflammatory cytokines in the peritoneal lavage fluid of mice with peritonitis, including IL-6, IFN-γ, IL-1β, NO, and TNF-α. Compared with the Control group, LPS treatment induced 4201 DEGs (2442 down-regulated DEGs and 1759 up-regulated DEGs). Compared with the LPS group, 30 DEGs were affected by TAK 242 (8 down-regulated DEGs and 22 up-regulated DEGs). A total of 10 TAK 242-triggered hub genes were obtained, and the possible docking modes between TAK 242 and hub proteins were acquired. Overall, our data demonstrated that a large number of DEGs were affected in LPS-triggered peritonitis mice. Moreover, the TLR4 inhibitor TAK 242 is capable of suppressing the inflammatory response of LPS-induced peritonitis. Our work provides clues for understanding the pathogenesis of LPS-induced peritonitis in mice. To complete the study, the researchers used (R)-Ethyl 6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-enecarboxylate (cas: 243984-11-4) .

(R)-Ethyl 6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-enecarboxylate(cas:243984-11-4) is a toll-like receptor 4 (TLR4) signaling inhibitor.Safety of (R)-Ethyl 6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-enecarboxylate And it can inhibits LPS-induced cytokine production in vitro (IC50 values are 1.3, 1.3 and 3.2 nM for IL-6, TNFα and NO production).

Reference:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Krief, Alain;Wouters, Johan;Norberg, Bernadette;Kremer, Adrian published 《Formalizing the mechanism of the allylic substitution reaction (SN’): application to the highly enantio- and diastereo-selective syntheses of 1-phenyl-2-vinylcyclopentanes》 in 2018. The article was appeared in 《ARKIVOC (Gainesville, FL, United States)》. They have made some progress in their research.Product Details of 39637-74-6 The article mentions the following:

An efficient stereoselective and high yielding syntheses of each of the four enantiomers of phenylcyclopentanes e.g., I bearing a quaternary center and a E-propenyl chain on the adjacent carbon that involves intramol. allylic substitution reactions has been reported. In complement to its synthetic value, this process models the SN’ reaction and allows prediction of its stereochem. outcome. The experimental procedure involved many compounds, such as (1S)-4,7,7-Trimethyl-3-oxo-2-oxabicyclo[2.2.1]heptane-1-carbonyl chloride(Chunks or pellets) (cas: 39637-74-6) .

(1S)-4,7,7-Trimethyl-3-oxo-2-oxabicyclo[2.2.1]heptane-1-carbonyl chloride(Chunks or pellets) (cas: 39637-74-6 Product Details of 39637-74-6) is a chiral derivatizing agent. It can be prepared by reacting (-)-(1S,4R)-camphanic acid with thionyl chloride.

Reference:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

del Hoyo, Ana M.;Latorre, Alfonso;Diaz, Raul;Urbano, Antonio;Carreno, M. Carmen published 《Enantiopure Helical Ferrocene-Triazole-Quinone Triads: Synthesis and Properties》 in 2015. The article was appeared in 《Advanced Synthesis & Catalysis》. They have made some progress in their research.Safety of (1S)-4,7,7-Trimethyl-3-oxo-2-oxabicyclo[2.2.1]heptane-1-carbonyl chloride(Chunks or pellets) The article mentions the following:

The Cu(I)-catalyzed (2+3) cycloaddition between azido (or azidomethylene) ferrocenes and enantiopure (P)-14-(p-ethynylphenyl)-[5]-tetrahydro-helicenequinone afforded ferrocene-triazole-quinone triads, whereas diazido ferrocenes gave rise to the corresponding double triads. In reactions with 1,1′-diazidoferrocene, the use of CuI/MeCN or CuSO₄/Na ascorbate/THF conditions allowed the divergent formation of an open chain dimeric structure or a 1,4-diaza-[4]-ferrocenophane resulting from an intramol. oxidative coupling of the two triazole units formed after the double cycloaddition process. The experimental procedure involved many compounds, such as (1S)-4,7,7-Trimethyl-3-oxo-2-oxabicyclo[2.2.1]heptane-1-carbonyl chloride(Chunks or pellets) (cas: 39637-74-6) .

(1S)-4,7,7-Trimethyl-3-oxo-2-oxabicyclo[2.2.1]heptane-1-carbonyl chloride(Chunks or pellets) (cas: 39637-74-6 Safety of (1S)-4,7,7-Trimethyl-3-oxo-2-oxabicyclo[2.2.1]heptane-1-carbonyl chloride(Chunks or pellets)) is a chiral derivatizing agent. It can be prepared by reacting (-)-(1S,4R)-camphanic acid with thionyl chloride.

Reference:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Recommanded Product: 243984-11-4In 2022, Zhang, Ruili;Liu, Qing;Guo, Rong;Zhang, Di;Chen, Yang;Li, Guangxing;Huang, Xiaodan published 《Selenium Deficiency Induces Autophagy in Chicken Bursa of Fabricius Through ChTLR4/MyD88/NF-kappaB Pathway》. 《Biological Trace Element Research》published the findings. The article contains the following contents:

To explore the role of ChTLR4/MyD88/NF-κB signaling pathway on autophagy induced by selenium (Se) deficiency in the chicken bursa of Fabricius, autophagosome formation in the bursa of Fabricius was observed by transmission electron microscopy. Quant. real-time PCR (qRT-PCR) and Western blot were used to detect the expression of ChTLR4 and its signaling pathway mols. (MyD88, TRIF, and NF-κB), inflammatory factors (IL-1β, IL-8, and TNF-α), and autophagy-related factors (ATG5, Beclin1, and LC3-II) in the Se-deficient chicken bursa of Fabricius at different ages. The results showed that ChTLR4/MyD88/NF-κB signaling pathway was activated in the chicken bursa of Fabricius and autophagy was induced at the same time by Se deficiency. In order to verify the relationship between the autophagy and ChTLR4/MyD88/NF-κB signaling pathway, HD11 cells were used to establish the normal C group, low Se group, and low Se + TLR4 inhibitor (TAK242) group. The results demonstrated that autophagy could be hindered when the TLR4 signaling pathway was inhibited under Se deficiency. Furthermore, autophagy double-labeled adenovirus was utilized to verify the integrity of autophagy flow induced by Se deficiency in HD11 cells. The results showed that it appeared to form a complete autophagy flow under the condition of Se deficiency and could be blocked by TAK242. In summary, we found that Se deficiency was involved in the chicken bursa of Fabricius autophagy occurring by activating the ChTLR4/MyD88/NF-κB pathway.(R)-Ethyl 6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-enecarboxylate (cas: 243984-11-4) were involved in the experimental procedure.

(R)-Ethyl 6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-enecarboxylate(cas:243984-11-4) can reduces lesion volume in a mouse model of cerebral cavernous malformations (CCMs).Recommanded Product: 243984-11-4 Also attenuates increased cytokine levels in a mouse sepsis model, when given in combination with ceftazidime. Cell permeable.

Reference:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics