Ali, Shaukat; Champagne, Danielle L.; Richardson, Michael K. published the artcile< Behavioral profiling of zebrafish embryos exposed to a panel of 60 water-soluble compounds>, Electric Literature of 6055-19-2, the main research area is locomotor behavior Danio embryo water soluble compound.
The zebrafish is a powerful whole animal model which is complementary to in vitro and mammalian models. It has been shown to be applicable to the high-throughput behavioral screening of compound libraries. We have analyzed 60 water-soluble toxic compounds covering a range of common drugs, toxins and chems., and representing various pharmacol. mechanisms. Wild-type zebrafish larvae were cultured individually in defined buffer in 96 well plates. They were exposed for a 96 h period starting at 24 h post fertilization (hpf). A logarithmic concentration series was used for range-finding, followed by a narrower geometric series for LC50 determination LC50 values were determined at 24 h intervals and behavioral testing was carried out on day 5. We used the visual motor response test, in which movement of individual larvae was analyzed using automated video-tracking. For all compounds, LC50 values were found to decrease as the embryo developed. The majority of compounds (57/60) produced an effect in both the basal (lights on) and challenge phases (lights off) of the behavioral assay. These effects were either (i) suppression of locomotor activity (monotonic concentration-response); (ii) stimulation then suppression (biphasic response); (iii) stimulation (monotonic response). We conclude that behavioral assays with zebrafish embryos could be useful for pharmaceutical efficacy and toxicity screening. The precise phenotypic outcome obtained with behavioral assay varies with compound class.
Behavioural Brain Research published new progress about Aquatic toxicity. 6055-19-2 belongs to class chlorides-buliding-blocks, and the molecular formula is C7H17Cl2N2O3P, Electric Literature of 6055-19-2.
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics