Harrill, Joshua A.; Freudenrich, Theresa; Wallace, Kathleen; Ball, Kenneth; Shafer, Timothy J.; Mundy, William R. published the artcile< Testing for developmental neurotoxicity using a battery of in vitro assays for key cellular events in neurodevelopment>, Formula: C7H17Cl2N2O3P, the main research area is primary cortical cell developmental neurotoxicity battery; Assay suite; Concentration-response; Developmental neurotoxicity; High content imaging; In vitro models; Toxicity screening.
Medium- to high-throughput in vitro assays that recapitulate the critical processes of nervous system development have been proposed as a means to facilitate rapid testing and identification of chems. which may affect brain development. In vivo neurodevelopment is a complex progression of distinct cellular processes. Therefore, batteries of in vitro assays that model and quantify effects on a variety of neurodevelopmental processes have the potential to identify chems. which may affect brain development at different developmental stages. In the present study, the results of concentration-response screening of 67 reference chems. in a battery of high content imaging and microplate reader-based assays that evaluate neural progenitor cell proliferation, neural proginitor cell apoptosis, neurite initiation/outgrowth, neurite maturation and synaptogenesis are summarized and compared. The assay battery had a high degree of combined sensitivity (87%) for categorizing chems. known to affect neurodevelopment as active and a moderate degree of combined specificity (71%) for categorizing chems. not associated with affects on neurodevelopment as inactive. The combined sensitivity of the assay battery was higher compared to any individual assay while the combined specificity of the assay battery was lower compared to any individual assay. When selectivity of effects for a neurodevelopmental endpoint as compared to general cytotoxicity was taken into account, the combined sensitivity of the assay battery decreased (68%) while the combined specificity increased (93%). The identity and potency of chems. identified as active varied across the assay battery, underscoring the need for use of a combination of diverse in vitro models to comprehensively screen chems. and identify those which potentially affect neurodevelopment. Overall, these data indicate that a battery of assays which address many different processes in nervous system development may be used to identify potential developmental neurotoxicants and to distinguish specific from generalized cytotoxic effects with a high degree of success.
Toxicology and Applied Pharmacology published new progress about Apoptosis. 6055-19-2 belongs to class chlorides-buliding-blocks, and the molecular formula is C7H17Cl2N2O3P, Formula: C7H17Cl2N2O3P.
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics