Tian, Zhenhao; Ding, Lele; Li, Kun; Song, Yunqing; Dou, Tongyi; Hou, Jie; Tian, Xiangge; Feng, Lei; Ge, Guangbo; Cui, Jingnan published the artcile< Rational Design of a Long-Wavelength Fluorescent Probe for Highly Selective Sensing of Carboxylesterase 1 in Living Systems>, Category: chlorides-buliding-blocks, the main research area is design long wavelength fluorescence sensing carboxylesterase 1.
Rational design of practical probes with excellent specificity and improved optical properties for a particular enzyme is always a big challenge. Herein, a practical and highly specific fluorescent probe for carboxylesterase 1 (CES1) was rationally designed using meso-carboxyl-BODIPY as the basic fluorophore based on the substrate preference and catalytic properties of CES1. Following mol. docking-based virtual screening combined with reaction phenotyping-based exptl. screening, we found that MMB (probe 7) exhibited the optimal combination of sensitivity and specificity toward human CES1 in contrast to other ester derivatives Under physiol. conditions, MMB could be readily hydrolyzed by CES1 and release MCB; such biotransformation brought great changes in the electronic properties at the meso position of the fluorophore and triggered a dramatic increase in fluorescence emission around 595 nm. Moreover, MMB was cell membrane permeable and was successfully applied to monitor the real activities of CES1 in various biol. samples including living cells, tissue slices, organs, and zebrafish. In summary, this study showed a good example for constructing specific fluorescent probe(s) for a target enzyme and also provided a practical and sensitive tool for real-time sensing of CES1 activities in complicated biol. samples. All these findings would strongly facilitate high-throughput screening of CES1 modulators and the studies on CES1-associated physiol. and pathol. processes.
Analytical Chemistry (Washington, DC, United States) published new progress about Danio rerio. 128-09-6 belongs to class chlorides-buliding-blocks, and the molecular formula is C4H4ClNO2, Category: chlorides-buliding-blocks.
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics