The author of 《Design, synthesis, and apoptosis-promoting effect evaluation of novel pyrazole with benzo[d]thiazole derivatives containing aminoguanidine units》 were Liu, Da Chuan; Gao, Mei Jia; Huo, Qiang; Ma, Tao; Wang, Ying; Wu, Cheng Zhu. And the article was published in Journal of Enzyme Inhibition and Medicinal Chemistry in 2019. Application In Synthesis of 3-Chlorobenzylchloride The author mentioned the following in the article:
New pyrazole with benzo[d]thiazoles containing hydrazinecarboximidamide substituent was synthesized and evaluated for cytotoxicity and apoptotic activity using the MTT assay, flow cytometry, and Western blot anal. Among the compounds studied, (E)-2-((1-(6-((4-fluorobenzyl)oxy)benzo[d]thiazol-2-yl)-3-phenyl-1H-pyrazol-4-yl)methylene) hydrazinecarboximidamide (8l) was potent, with IC50 values of 2.41 μM, 2.23 μM, 3.75 μM and 2.31 μM in vitro anti-proliferative activity testing against triple-neg. breast cancer cell line MDA-MB-231, non-triple-neg. breast cancer MCF-7 cells, and human hepatocarcinoma HepG2 cells, and SMMC-7721 cells, resp. Especially, the activity against MDA-MB-231 was similar to that of Doxorubicin, which was used as a pos. control in this study. Next, the Annexin V/PI flow cytometry assay was used at different concentrations of compound 8l to demonstrate that compound 8l induced apoptosis of MDA-MB-231 cells in a concentration-dependent manner. Finally, these results were further verified by Western blot anal. Taken together, the results of this study revealed that compound 8l may be a potential anticancer compound play a significant role in the subsequent researches. After reading the article, we found that the author used 3-Chlorobenzylchloride(cas: 620-20-2Application In Synthesis of 3-Chlorobenzylchloride)
3-Chlorobenzylchloride(cas: 620-20-2) has been used in the reaction of 3-methoxybenzyl chloride and ethyl 4-bromobenzoate in pure water, using zinc dust and a Pd catalyst.Application In Synthesis of 3-Chlorobenzylchloride
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics