Thines, Eckhard; Daussmann, Thomas; Semar, Martin; Sterner, Olov; Anke, Heidrun published their research in Zeitschrift fuer Naturforschung, C: Biosciences on December 31 ,1995. The article was titled 《Fungal melanin biosynthesis inhibitors: introduction of a test system based on the production of dihydroxynaphthalene (DHN) melanin in agar cultures》.Reference of 3,5-Dichloro-4-methoxybenzoic acid The article contains the following contents:
In a screening for inhibitors of fungal melanin biosynthesis a novel test system was successfully employed. With this test system, based on the production of dihydroxynaphthalene melanin (DHN melanin) by a Lachnellula species in agar cultures, extracts of cultures of basidiomycetes, ascomycetes and deuteromycetes were tested. From fermentation of Collybia dryophila, 6-methylpurine and 6-methyl-9-β-D-ribofuranosyl purine were isolated as the active principles and 3,5-dichloro-4-methoxybenzoic acid (I) was obtained from cultures of Stropharia squamosa. The corresponding alc. (II) and aldehyde (III) previously isolated from several basidiomycetes were also active in this test system. In a screening of fungal metabolites, 1-methoxy-8-hydroxynaphthalene and 1,8-dimethoxynaphthalene inhibited biosynthesis of DHN melanin. Among the inhibitors identified, compounds I-III were the most selective ones. They were the only metabolites without cytotoxic activities. In the experiment, the researchers used many compounds, for example, 3,5-Dichloro-4-methoxybenzoic acid(cas: 37908-97-7Reference of 3,5-Dichloro-4-methoxybenzoic acid)
3,5-Dichloro-4-methoxybenzoic acid(cas: 37908-97-7) belongs to organochlorine compounds. Alkanes and aryl alkanes may be chlorinated under free radical conditions, with UV light. However, the extent of chlorination is difficult to control. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst.Reference of 3,5-Dichloro-4-methoxybenzoic acid
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics