Sakura, Mizuaki; Masuda, Hitoshi; Matsukoa, Yoh; Yokoyama, Minato; Kawakmi, Satoru; Kihara, Kazunori published the artcile< Rolipram, a specific type-4 phosphodiesterase inhibitor, inhibits cyclophosphamide-induced haemorrhagic cystitis in rats>, Application In Synthesis of 6055-19-2, the main research area is rolipram phosphodiesterase inhibitor cyclophosphamide monohydrate hemorrhagic cystitis iNOS antiinflammatory.
To investigate the protective roles of type 4 phosphodiesterase (PDE4) inhibitor in cyclophosphamide (CYP)-induced hemorrhagic cystitis, as the PDE4 inhibitor has anti-inflammatory effects but its characterization is still unknown in urinary tract diseases. In female Sprague-Dawley rats, CYP was administered i.p. and bladders were harvested 24 h after CYP injection. In another group, rolipram as a PDE4 inhibitor was administered before CYP treatment. The effects and mechanisms of CYP with/ without rolipram pretreatment were evaluated by microscopic features, bladder wet weight, myeloperoxidase (MPO) activity, nitric oxide (NO)-metabolite production and expression levels of inflammation-related genes. CYP injection resulted in severe cystitis. Pretreatment with rolipram significantly reduced the increase in bladder wet weight and MPO activity, and ameliorated histol. inflammatory changes caused by CYP. The levels of inflammation-related transcripts including inducible NO synthase (iNOS), interleukin-1β and tumor necrosis factor-α, induced by CYP, were down-regulated significantly by pretreatment with rolipram. Also, rolipram reduced the NO-metabolite production and iNOS protein expression in the immunohistochem. examination These results indicate that rolipram can attenuate the development of CYP-induced cystitis in rats by suppressing cytokine production and iNOS induction. Thus, treatment with PDE4 inhibitor has potential clin. implications of the prevention of bladder inflammatory diseases.
BJU International published new progress about Anti-inflammatory agents. 6055-19-2 belongs to class chlorides-buliding-blocks, and the molecular formula is C7H17Cl2N2O3P, Application In Synthesis of 6055-19-2.
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics