Rezaei, Elham Babazadeh; Abedinifar, Fahimeh; Azizian, Homa; Montazer, Mohammad Nazari; Asadi, Mehdi; Hosseini, Samanesadat; Sepehri, Saghi; Mohammadi-Khanaposhtani, Maryam; Biglar, Mahmood; Larijani, Bagher; Amanlou, Massoud; Mahdavi, Mohammad published their research in Chemical Papers in 2021. The article was titled 《Design, synthesis and evaluation of metronidazole-1,2,3-triazole derivatives as potent urease inhibitors》.Formula: C7H6BrCl The article contains the following contents:
A new series of benzyl-(imidazolylmethyl)triazole derivatives I [R = H, 2-Me, 4-Br, etc.] was synthesized and evaluated as Helicobacter pylori urease inhibitors. All the synthesized compounds were more potent than standard inhibitor thiourea against urease. Among the synthesized compounds, compound I [R = 4-F] (IC50 = 1.975 ± 0.25μM) with inhibitory activity around 11-fols more than thiourea (IC50 = 22.00 ± 0.14μM) was the most potent compound Kinetic study of this compound revealed that compound I [R = 4-F] inhibited urease in an uncompetitive mode. Based on mol. modeling study, compound I [R = 4-F] pointed toward the bi-nickel center and stabilized by H-bond and T-shape π-π hydrophobic interactions with the critical residues His492 and Asp633. Moreover, it anchored to the helix-turn-helix motif in the active site cavity through interaction with His593 and Arg609. Consequently, it proposed that compound I [R = 4-F] through stabilization of active site flap inhibited urease activity. After reading the article, we found that the author used 1-(Bromomethyl)-4-chlorobenzene(cas: 622-95-7Formula: C7H6BrCl)
1-(Bromomethyl)-4-chlorobenzene(cas: 622-95-7) may be used to synthesize 1-(4-chlorobenzyl)-2-(pyrrolidin-1-yl-methyl)-1H-benzimidazole dihydrochloride.Formula: C7H6BrCl It can be synthesized by reacting 4-chlorobenzyl alcohol with bromodimethylsulfonium bromide (BDMS) It can also be synthesized by refluxing a mixture of 4-chlorobenzaldehyde, chlorotrimethylsilane, 1,1,3,3-tetramethyldisiloxane and lithium bromide.
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics