Akintola, Oluwafemi; Ren, Weiwu; Adabala, Pal John Pal; Bhosale, Sandeep; Wang, Yang; Ganga-Sah, Yumeela; Britton, Robert; Bennet, Andrew J. published their research in ACS Catalysis on August 6 ,2021. The article was titled 《Intrinsic Nucleophilicity of Inverting and Retaining Glycoside Hydrolases Revealed Using Carbasugar Glyco-Tools》.Category: chlorides-buliding-blocks The article contains the following contents:
Hydrolyzes of cyclohexenyl-based carbasugars that mimic either α-D-glucose or α-D-galactose were explored with two Bacteroides thetaiotaomicron enzymes from glycoside hydrolase family 97: an inverting α-glucosidase (BtGH97a) and a retaining α-galactosidase (BtGH97b). Specifically, the kinetic data for the inverting α-glucosidase is consistent with the reaction giving a hydrolyzed inverted carbaglucose product by a mechanism that proceeds with little nucleophilic participation by the bound water mol. at the reaction transition state. The enzymic rate constant ratio for the Ph carbasugars contrasts with the corresponding kinetic data obtained using natural substrate Ph glycopyranosides. This modest difference in rate constants underscores our conclusion that retaining glycoside hydrolases may not have optimized the nucleophilicity of their active site nucleophiles with the result that the transition state free energies for formation and hydrolysis of the covalent enzyme intermediate are matched. After reading the article, we found that the author used 3-Chloro-4-fluoronitrobenzene(cas: 350-30-1Category: chlorides-buliding-blocks)
3-Chloro-4-fluoronitrobenzene(cas: 350-30-1) belongs to organochlorine compounds. The wide structural variety and divergent chemical properties of organochlorides lead to a broad range of names, applications, and properties.Category: chlorides-buliding-blocks Organochlorine compounds have wide use in many applications, though some are of profound environmental concern, with TCDD being one of the most notorious.
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics