The author of 《Sensing carboxylesterase 1 in living systems by a practical and isoform-specific fluorescent probe》 were Ding, Lele; Tian, Zhenhao; Hou, Jie; Dou, Tongyi; Jin, Qiang; Wang, Dandan; Zou, Liwei; Zhu, Yadi; Song, Yunqing; Cui, Jingnan; Ge, Guangbo. And the article was published in Chinese Chemical Letters in 2019. Application of 5781-53-3 The author mentioned the following in the article:
Carboxylesterase 1 (CES1), one of the most abundant serine hydrolases in mammals, has drawn much attentions in recent years, owing to this enzyme involves in many physiol. processes via hydrolysis of both endogenous esters and xenobiotic esters. Herein, to real-time monitor the activities of CES1 in various biol. systems, a practical and isoform-specific fluorescent probe was developed on the basis of the substrate preference of CES1, as well as the structural and optical properties of BODIPY dyes. After screening of a panel of BODIPY ester derivatives, probe 1 displayed the best combination of specificity, sensitivity, enzymic kinetics and applicability for monitoring CES1 activities in real samples. This probe was successfully used to detect CES1 activities in several biol. systems including tissue preparations, living cells, tissue slices and zebrafish. Furthermore, the biomedical applications of probe 1 for screening of CES1 inhibitors were also demonstrated using tissue preparations or living cells as enzyme sources. In summary, a practical and broadly applicable tool for real-time monitoring CES1 in biol. systems was developed and well-characterized, which held great promise for further investigations on CES1-associated drug discovery, clin. practice and fundamental research. In addition to this study using Methyl 2-chloro-2-oxoacetate, there are many other studies that have used Methyl 2-chloro-2-oxoacetate(cas: 5781-53-3Application of 5781-53-3) was used in this study.
Methyl 2-chloro-2-oxoacetate(cas: 5781-53-3) belongs to acyl chlorides. In the laboratory, acyl chlorides are generally prepared by treating carboxylic acids with thionyl chloride (SOCl2). The reaction is catalyzed by dimethylformamide and other additives.Application of 5781-53-3
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics