Takeshita, Kenji; Ogawa, Hiroaki I.; Maeda, Toshinari published the artcile< Structural chromosome aberrations cause swelling of the nucleus>, HPLC of Formula: 6055-19-2, the main research area is clastogen chromosomal aberration nuclear swelling; Aneugen; Carcinogen; Chromosomal aberration; Clastogen; Genotoxicity; Non-mutagen; Nuclear swelling.
Background: Carcinogens are known to cause swelling of the mammalian cell nucleus. However, the mechanism of the swelling and its toxicol. significance have not been fully elucidated. Since nuclear swelling (NS hereafter) has been frequently observed in chromosomal aberration (CA hereafter) tests (in vitro), the relationship between NS and CAs was investigated in this study. Results: In a short-term CA test using the fibroblast CHL cell line, the appearance of NS increased in a dose-dependent manner after exposure to six types of clastogens (mitomycin C, Me methane sulfonate, 1-methyl-3-nitro-1-nitrosoguanidine, benzo[a]pyrene, cyclophosphamide monohydrate, and 9,10-dimethyl-2-benzanthracene), and a strong correlation was found between NS (%) and CAs (%) at each dosage. Therefore, we hypothesized that clastogens cause NS in cultured mammalian cells, since the mouse lymphoma L5178Y cell line is known to have a similar sensitivity to clastogens. Thus, we measured NS for 14 compounds (clastogens) that are known to induce structural CAs, 4 aneugens, and 12 non-mutagenes. Almost all clastogens caused NS of more than 5%, which increased in a dose-dependent manner. Among the aneugens, colchicine, and diethylstilbestrol caused the same level of NS % as the clastogens, while carbendazim and trichlorfon caused a similar level of NS % as the clastogens only at higher levels of cytotoxicity. Almost all the non-mutagens caused less than 5% NS. Conclusions: These results strongly suggest that NS is mainly caused by structural aberrations in the nucleus during interphase of the cell cycle.
Genes and Environment published new progress about Aneugens. 6055-19-2 belongs to class chlorides-buliding-blocks, and the molecular formula is C7H17Cl2N2O3P, HPLC of Formula: 6055-19-2.
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics