On February 21, 2020, Adams, Casey J.; Krueger, Ruby; Meade, Thomas J. published an article.Quality Control of tert-Butyl trichloroacetimidate The title of the article was A Multimodal Ca(II) Responsive Near IR-MR Contrast Agent Exhibiting High Cellular Uptake. And the article contained the following:
Ca(II) ions are critical for the proper function of neurons by contributing to synaptic signaling and regulating neuronal plasticity. Dysregulation of Ca(II) is associated with a number of pathologies that cause neurodegeneration; therefore the ability to monitor Ca(II) intracellularly is an important target for mol. imaging. Contrast-enhanced MR imaging is a promising modality for imaging changes in Ca(II) concentrations However, the majority of Ca(II) responsive MR agents are limited to the extracellular space or hindered by poor cellular uptake. Here, we describe a new class of multimodal, bioresponsive Ca(II) magnetic resonance agents that are coupled to the NIR probe IR-783. This new design is based on previous generations of our Ca(II) MR agents but overcomes two significant challenges: (1) the presence of the NIR probe dramatically increases cellular uptake of the agent and (2) provides histol. validation of the MR signal using NIR fluorescence imaging. IR-783 targets organic anion transporter polypeptides, and we demonstrate that the agents are not toxic in HT-22 or U-87 MG cells up to 20μM. The cellular uptake of complex 1 was measured to be greater than 16 fmol per cell (where ∼1 fmol/cell is detectable in acquired MR images). Complex 1 is simultaneously detectable by both MR and NIR fluorescence imaging in vitro and is activated (turned on) by intracellular Ca(II) at concentrations between 1 and 10μM. The experimental process involved the reaction of tert-Butyl trichloroacetimidate(cas: 98946-18-0).Quality Control of tert-Butyl trichloroacetimidate
The Article related to calcium nearir mr contrast agents preparation cancer imaging, Radiation Biochemistry: Disease Diagnosis and Therapy and other aspects.Quality Control of tert-Butyl trichloroacetimidate
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