On July 20, 2020, Baalmann, Mathis; Neises, Laura; Bitsch, Sebastian; Schneider, Hendrik; Deweid, Lukas; Werther, Philipp; Ilkenhans, Nadja; Wolfring, Martin; Ziegler, Michael J.; Wilhelm, Jonas; Kolmar, Harald; Wombacher, Richard published an article.Category: chlorides-buliding-blocks The title of the article was A Bioorthogonal Click Chemistry Toolbox for Targeted Synthesis of Branched and Well-Defined Protein-Protein Conjugates. And the article contained the following:
Bioorthogonal chem. holds great potential to generate difficult-to-access protein-protein conjugate architectures. Current applications are hampered by challenging protein expression systems, slow conjugation chem., use of undesirable catalysts, or often do not result in quant. product formation. Here the authors present a highly efficient technol. for protein functionalization with commonly used bioorthogonal motifs for Diels-Alder cycloaddition with inverse electron demand (DAinv). With the aim of precisely generating branched protein chimeras, the authors systematically assessed the reactivity, stability and side product formation of various bioorthogonal chemistries directly at the protein level. The authors demonstrate the efficiency and versatility of the authors′ conjugation platform using different functional proteins and the therapeutic antibody trastuzumab. This technol. enables fast and routine access to tailored and hitherto inaccessible protein chimeras useful for a variety of scientific disciplines. The authors expect the authors′ work to substantially enhance antibody applications such as immunodetection and protein toxin-based targeted cancer therapies. The experimental process involved the reaction of tert-Butyl trichloroacetimidate(cas: 98946-18-0).Category: chlorides-buliding-blocks
The Article related to bioorthogonal click chem branched protein conjugate, antibody-drug conjugates, bioorthogonal chemistry, click chemistry, protein ligation, protein-protein conjugates, Placeholder for records without volume info and other aspects.Category: chlorides-buliding-blocks
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics