Fisch, Alexander; Reiber, Chelsea; Schroeder, William; Smart, Robert; Patel, Osman V. published an article in 2020, the title of the article was Potent suppression of proliferation of breast carcinoma cells by a novel anthranilic acid derivative.Formula: C7H6ClNO2 And the article contains the following content:
Chemotherapy remains the mainstay therapy for the most intrusive-type of breast carcinoma, triple-neg. breast cancer (TNBC) that has a higher tendency for visceral metastases, relapses, and poor prognoses. It is well recognized that the reactivation of a ribonucleoprotein enzyme, telomerase is among the key determinants of breast carcinogenesis, cellular immortalization and metastatic progression. Therefore, our objectives were to assess (i) short- and (ii) long-term effects of a novel anthranilic acid (GV6) developed at our institute and compare it to a known analog, BIBR1532 on TNBC (MDA-MB 231) and non-TNBC (MCF-7) cells. Seeded TNBC and non-TNBC flasks were supplemented with 25 μM of either BIBR 1532 or GV6 or solvent (DMSO) alone for 14 (Short-term) or 27 (Longterm) days. Trypan-blue dye exclusion test was utilized to determine viable cells, senescence-associated β-galactosidase activity was employed to detect senescent cells and qPCR was used to quantitate transcript abundance. Cell viability assay revealed that short- and longterm growth inhibitions of TNBC and non-TNBC cells were comparable between BIBR1532 and GV6. Cytochem. detection of β-Galactosidase demonstrated that GV6 was equally effective in inducing replicative senescence in treated TNBC and non-TNBC cells. Short- and long-term regimen of BIBR1532 and GV6 showed similar druginduced downregulation of hTERT in TNBC and non-TNBC cells. Results indicate that GV6 is an equally potent inhibitor of hTERT that induces growth impedance and triggers senescence in TNBC, as well as non-TNBC cells and merits further studies for improved treatment options. The experimental process involved the reaction of 2-Amino-4-chlorobenzoic acid(cas: 89-77-0).Formula: C7H6ClNO2
The Article related to cell proliferation breast carcinoma anthranilic acid derivative drug development, Placeholder for records without volume info and other aspects.Formula: C7H6ClNO2
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