Guo, Yuchuan; Wang, Lizhen; Guo, Jiatong; Gu, Guofeng; Guo, Zhongwu published an article in 2017, the title of the article was Biochemical studies of inositol N-acetylglucosaminyltransferase involved in mycothiol biosynthesis in Corynebacterium diphtheria.Computed Properties of 14602-86-9 And the article contains the following content:
Mycothiol (MSH) is the predominant low mol. weight thiol produced by actinomycetes, and it plays a pivotal role in the bacterial detoxication process. 1L-myo-Inositol-1-phosphate (1L-Ins-1-P) α-N-acetylglucosaminyltransferase (GlcNAc-T), known as MshA, is the only glycosyltransferase involved in MSH biosynthesis. In this work, the MshA from Corynebacterium diphtheria, named as CdMshA, was expressed, purified, and studied in detail. Its enzymic activity to transfer GlcNAc to 1L-Ins-1-P was confirmed by the isolation and rigorous characterization of its reaction product 3-phospho-1-D-myo-inositol-2-acetamido-2-deoxy-α-D-glucopyranoside. CdMshA was shown to accept only UDP-GlcNAc and 1L-Ins-1-P as its substrates among various tested glycosyl donors, such as UDP-GlcNAc, UDP-Gal, UDP-Glc, UDP-GalNAc and UDP-GlcA, and glycosyl acceptors, such as myo-inositol, 1L-Ins-1-P and 1D-Ins-1-P. The results have demonstrated the strict substrate selectivity of CdMshA. Furthermore, its reaction kinetics with UDP-GlcNAc and 1L-Ins-1-P as substrates were characterized, while site-directed mutagenesis of CdMshA disclosed that its amino acid residues N28, K81 and R157 were essential for its enzymic activity. The experimental process involved the reaction of (1R,2S,5R)-2-Isopropyl-5-methylcyclohexyl carbonochloridate(cas: 14602-86-9).Computed Properties of 14602-86-9
The Article related to corynebacterium purification inositol phosphate acetylglucosaminyltransferase msha substrate selectivity, Enzymes: Separation-Purification-General Characterization and other aspects.Computed Properties of 14602-86-9
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics