Stanley, Mathew; Virdee, Satpal published an article in 2016, the title of the article was Genetically Directed Production of Recombinant, Isosteric and Nonhydrolysable Ubiquitin Conjugates.Recommanded Product: tert-Butyl trichloroacetimidate And the article contains the following content:
We describe the genetically directed incorporation of aminooxy functionality into recombinant proteins by using a mutant Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNACUA pair. This allows the general production of nonhydrolysable ubiquitin conjugates of recombinant origin by bioorthogonal oxime ligation. This was exemplified by the preparation of nonhydrolysable versions of diubiquitin, polymeric ubiquitin chains and ubiquitylated SUMO. The conjugates exhibited unrivalled isostery with the native isopeptide bond, as inferred from structural and biophys. characterization. Furthermore, the conjugates functioned as nanomolar inhibitors of deubiquitylating enzymes and were recognized by linkage-specific antibodies. This technol. should provide a versatile platform for the development of powerful tools for studying deubiquitylating enzymes and for elucidating the cellular roles of diverse polyubiquitin linkages. The experimental process involved the reaction of tert-Butyl trichloroacetimidate(cas: 98946-18-0).Recommanded Product: tert-Butyl trichloroacetimidate
The Article related to genetically recombinant isosteric nonhydrolysable ubiquitin conjugate, genetic code expansion, isopeptide, oxime, polymerization, synthetic methods, ubiquitylation, Placeholder for records without volume info and other aspects.Recommanded Product: tert-Butyl trichloroacetimidate
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