Esteve-Turrillas, F. A.; Mercader, J. V.; Agullo, C.; Abad-Somovilla, A.; Abad-Fuentes, A. published an article in 2017, the title of the article was A class-selective immunoassay for simultaneous analysis of anilinopyrimidine fungicides using a rationally designed hapten.SDS of cas: 35444-44-1 And the article contains the following content:
The development of multianalyte immunoassays constitutes a main research issue in the field of bioanal. techniques. In the present study, class-specific antibodies against the three members of the anilinopyrimidine family of fungicides (pyrimethanil, cyprodinil and mepanipyrim) were raised by using a bioconjugate of a rationally designed hapten [5-(6-methyl-2-(phenylamino)pyrimidin-4-yl)pentanoic acid]. Highly sensitive immunoassays were developed for the generic determination of these compounds, using the competitive ELISA (ELISA). Particularly, a direct antibody-coated competitive ELISA afforded identical sensitivity for the three anilinopyrimidines, with IC50 values of 0.26, 0.27 and 0.25 μg L-1 for pyrimethanil, cyprodinil and mepanipyrim, resp. This immunoassay was fully characterized and applied to the multianalyte determination of anilinopyrimidine fungicides in white and red wines, with a limit of quantification of 1 μg L-1, average recoveries from 93.1 to 114.4%, and relative standard deviations lower than 20%. Com. wine samples were analyzed and those containing detectable anilinopyrimide residues were verified by a reference chromatog. technique. The experimental process involved the reaction of Methyl 6-chloro-6-oxohexanoate(cas: 35444-44-1).SDS of cas: 35444-44-1
The Article related to anilinopyrimidine fungicide immunoassay hapten, Food and Feed Chemistry: Analysis and other aspects.SDS of cas: 35444-44-1
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