On December 14, 2016, Li, Jinbo; Huang, Lei; Xiao, Xiao; Chen, Yingjie; Wang, Xingxing; Zhou, Zhengquan; Zhang, Chenyu; Zhang, Yan published an article.Computed Properties of 35444-44-1 The title of the article was Photoclickable MicroRNA for the Intracellular Target Identification of MicroRNAs. And the article contained the following:
MicroRNAs (miRNAs) are important gene regulators that bind with target genes and repress target gene expression at post-transcriptional level. The identification of target genes associated with miRNAs inside different cells is of major challenge in miRNA chem.-biol. due to lack of functional miRNAs bearing appropriate tags. Here we report photo-clickable miRNAs as appropriately pre-tagged miRNAs that keep the intracellular function of miRNAs and allow the addition of mol. handles through photo-click reaction. The photo-clickable miRNAs upon transfection inside cells were able to form functional complexes with target genes and repress target gene expression. Target genes associated with the photo-clickable miRNA in the complex were then tagged with the mol. handle through photo-click reaction for pull-down and identification. Using photo-clickable miR-106a, miR-27 and miR-122, we firstly verified that their intracellular function was comparable to intact miRNAs, which showed obvious advantage over corresponding biotinylated miRNAs. After attaching the biotin handle to the associated complex containing the photo-clickable miRNAs through the tetrazole-ene photo-click reaction, target genes previously bound with these miRNAs inside cells were successfully pulled town and analyzed. The application of this strategy was demonstrated by the identification of several new target genes of miR-122, followed by revealing a novel regulatory pathway in HepG2 cells with regard to the role of PEG10 in miR-122-promoted cell apoptosis. The experimental process involved the reaction of Methyl 6-chloro-6-oxohexanoate(cas: 35444-44-1).Computed Properties of 35444-44-1
The Article related to photoclickable microrna target gene identification hela hepg2, Biochemical Genetics: Methods and other aspects.Computed Properties of 35444-44-1
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