Yamamura, Ei-Tora published the artcileA novel method of producing the pharmaceutical intermediate (R)-2-chloromandelic acid by bioconversion, Safety of (S)-Methyl 2-(2-chlorophenyl)-2-hydroxyacetate, the main research area is chloromandelic Exophiala dermatitidis hydrolysis bioconversion; 2-chloromandelic acid; Asymmetric hydrolysis; bioconversion; esterase; expression.
(R)-2-Chloromandelic acid (RCM) is one of the chiral building blocks used in the pharmaceutical industry. As a result of screening for microorganisms that asym. hydrolyze racemic 2chloromandelic acid Me ester (CMM), Exophiala dermatitidis NBRC6857 was found to produce RCM at optical purity of 97% ee. The esterase that produces RCM, EstE, was purified from E. dermatitidis NBRC6857, and the optimal temperature and pH of EstE were 30°C and 7.0, resp. The estE gene that encodes EstE was isolated and overexpressed in Escherichia coli JM109. The activity of recombinant E. coli JM109 cells overexpressing estE was 553 times higher than that of E. dermatitidis NBRC6857. RCM was produced at conversion rate of 49% and at optical purity of 97% ee from 10% CMM with 0.45 mg-dry-cell/L recombinant E. coli JM109 cells. Based on these findings, RCM production by bioconversion of CMM may be of interest for future industrial applications.
Bioscience, Biotechnology, and Biochemistry published new progress about Escherichia coli. 32345-60-1 belongs to class chlorides-buliding-blocks, name is (S)-Methyl 2-(2-chlorophenyl)-2-hydroxyacetate, and the molecular formula is C9H9ClO3, Safety of (S)-Methyl 2-(2-chlorophenyl)-2-hydroxyacetate.
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics