Multi-faceted Set-up of a Diverse Ketoreductase Library Enables the Synthesis of Pharmaceutically-relevant Secondary Alcohols was written by Voss, Moritz;Kueng, Robin;Hayashi, Takahiro;Jonczyk, Magdalena;Niklaus, Michael;Iding, Hans;Wetzl, Dennis;Buller, Rebecca. And the article was included in ChemCatChem in 2021.Product Details of 638-07-3 The following contents are mentioned in the article:
Enzymes are valuable tools to introduce chirality into small mols. Especially, ketoreductase (KRED)-catalyzed transformations of ketones to yield chiral secondary alcs. have become an established biocatalytic process step in the pharmaceutical and fine chem. industry. Development time, however, remains a critical factor in chem. process development and thus, the competitiveness of a biocatalytic reaction step is often governed by the availability of off-the-shelf enzyme libraries. To expand the biocatalytic toolbox with addnl. ketoreductases, we established a multi-faceted screening procedure to capture KRED diversity from different sources, such as literature, available genome data, and uncharacterized microbial strains. Overall, we built a library consisting of 51 KRED enzymes, 29 of which have never been described in literature before. Notably, 18 of the newly described enzymes exhibited anti-Prelog preference complementing the majority of ketoreductases which generally follow Prelog’s rule. Anal. of the library’s catalytic activity toward a chem. diverse ketone substrate set of pharmaceutical interest further highlighted the broad substrate scope and the complementing enantio-preference of the individual KREDs. Using the generated sequence-function data of the included short chain dehydrogenases in a bioinformatic anal. led to the identification of possible sequence determinants of the stereospecificity exhibited by these enzymes. This study involved multiple reactions and reactants, such as Ethyl 4-chloro-3-oxobutanoate (cas: 638-07-3Product Details of 638-07-3).
Ethyl 4-chloro-3-oxobutanoate (cas: 638-07-3) belongs to organic chlorides. Organic chlorides can be used in production of: PVC, pesticides, chloromethane, teflon, insulators. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst.Product Details of 638-07-3
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics