A novel electrochemical aptasensor for serum dopamine detection based on methylene blue-integrated m-PdNFs signal material was written by Zhang, Chengli;You, Xun;Li, Yuliang;Zuo, Yuanyuan;Wang, Wei;Li, DanDan;Huang, ShengWei;Hu, Hangzhi;Yuan, Feng;Shao, Fuqiang;Yin, Minggang. And the article was included in Sensors and Actuators, B: Chemical in 2022.Synthetic Route of Cl4Na2Pd The following contents are mentioned in the article:
In this work, an aptamer was used as an identifying mol. and a novel amplification strategy based on Ce-MOF and m-PdNFs-G4-MBs for the sensitive detection of dopamine (DA) is demonstrated. Notably, a novel dopamine aptamer with higher affinity and specificity was chosen, and the PdNF was functionalized with MB via embeddedness and surface decoration for enhancing the intensity of electrochem. signal. The synthesized m-PdNFs-G4-MBs nanocomposite not only acts as the electrochem. indicator but also to label single-stranded DNA (S2) to acquire signaling probes (m-PdNFs-S2-G4-MBs) as a nanocarrier. Besides, an -NH2 modified single-strand DNA (S1) is designed, and form an S1-AP double-strand by annealing reaction with aptamer (AP), then the S1-AP is fixed on the Ce-MOF surface, decorated on electrode. In the presence of DA, DA bind to the AP and S1 regain single-stranded structure. And then, the single-stranded S1 recognizes m-PdNFs-S2-G4-MBs and captures them, ultimately producing an enhanced elec. signal. Due to the extraordinary conductive performance of Ce-MOF and the signal amplification of m-PdNFs-S2-G4-MBs, the new detection platform achieves a min. detection limit to 6 pmol/L for DA and with a good specificity. Prominently, the results obtained from our platform for serum DA detection are highly consistent with the UPLC-MS method. These results suggest that this new platform has the potential to provide a convenient and efficient tool for the diagnosis of multiple neurol. disorders in the future. This work also provides a good strategy for the construction of other biomol. sensors. This study involved multiple reactions and reactants, such as Sodium tetrachloropalladate(II) (cas: 13820-53-6Synthetic Route of Cl4Na2Pd).
Sodium tetrachloropalladate(II) (cas: 13820-53-6) belongs to organic chlorides. Chlorination modifies the physical properties of hydrocarbons in several ways. These compounds are typically denser than water due to the higher atomic weight of chlorine versus hydrogen.While alkyl bromides and iodides are more reactive, alkyl chlorides tend to be less expensive and more readily available. Alkyl chlorides readily undergo attack by nucleophiles.Synthetic Route of Cl4Na2Pd
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics