Electronic and Steric Optimization of Fluorogenic Probes for Biomolecular Imaging was written by Chyan, Wen;Kilgore, Henry R.;Gold, Brian;Raines, Ronald T.. And the article was included in Journal of Organic Chemistry in 2017.Application of 95-88-5 The following contents are mentioned in the article:
Fluorogenic probes are invaluable tools for spatiotemporal studies within live cells. In common fluorogenic probes, the intrinsic fluorescence of a small-mol. fluorophore is masked by esterification until entry into a cell, where endogenous esterases catalyze the hydrolysis of the masking groups, generating fluorescence. The susceptibility of masking groups to spontaneous hydrolysis is a major limitation of these probes. Previous attempts to address this problem have incorporated auto-immolative linkers at the cost of atom economy and synthetic adversity. Here, the authors report on a linker-free strategy that employs adventitious electronic and steric interactions in easy-to-synthesize probes. X···C:O n→π* interactions and acyl group size are optimized in 2′,7′-dichlorofluorescein diisobutyrate. This probe is relatively stable to spontaneous hydrolysis but is a highly reactive substrate for esterases both in vitro and in cellulo, yielding a bright, photostable fluorophore with utility in biomol. imaging. This study involved multiple reactions and reactants, such as 4-Chlororesorcinol (cas: 95-88-5Application of 95-88-5).
4-Chlororesorcinol (cas: 95-88-5) belongs to organic chlorides. Organic chlorides are compounds containing a carbon-chlorine bond, which are widely used in the oil field as a wax dissolver. While alkyl bromides and iodides are more reactive, alkyl chlorides tend to be less expensive and more readily available. Alkyl chlorides readily undergo attack by nucleophiles.Application of 95-88-5
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics