Significantly enhancing the biocatalytic synthesis of chiral alcohols by semi-rationally engineering an anti-Prelog carbonyl reductase from Acetobacter sp. CCTCC M209061 was written by Wei, Ping;Guo, Ze-Wang;Wu, Xiao-Ling;Liang, Shan;Ou, Xiao-Yang;Xu, Pei;Zong, Min-Hua;Yang, Ji-Guo;Lou, Wen-Yong. And the article was included in Molecular Catalysis in 2019.Computed Properties of C6H9ClO3 The following contents are mentioned in the article:
Chiral alcs. and their derivatives are vital building blocks to synthesize pharmaceutical drugs and high-valued chems. Wild-type carbonyl reductase AcCR from Acetobacter sp. has ideal enantioselectivity toward 11 prochiral substrates (e.e.>99%) but poor activity. In this work, a semi-rational engineering was performed to enhance the activity of AcCR. Fortunately, three pos. double-mutants (mut-E144A/G152L, mut-G152L/Y189 N, and mut-I147 V/G152L) with specific activity 17-61 folds higher than that of enzyme without modified were achieved. Kinetic studies suggested that the catalytic efficiencies (kcat/Km) of these mutants were also well enhanced. Finally, these modified mut-AcCRs were successfully applied in asym. reductions of 11 structurally diverse prochiral substrates (200 mM) with excellent product yields (76.8%-99.1%) and enantiomeric excess (e.e.>99%), which provides an alternative strategy for efficient synthesis of chiral alcs. for pharmaceuticals industry with ideal yield and enantioselectivity. This study involved multiple reactions and reactants, such as Ethyl 4-chloro-3-oxobutanoate (cas: 638-07-3Computed Properties of C6H9ClO3).
Ethyl 4-chloro-3-oxobutanoate (cas: 638-07-3) belongs to organic chlorides. Organic chlorides can be used in production of: PVC, pesticides, chloromethane, teflon, insulators. The haloform reaction, using chlorine and sodium hydroxide, is also able to generate alkyl halides from methyl ketones, and related compounds. Chloroform was formerly produced thus.Computed Properties of C6H9ClO3
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics