An activatable ratiometric near-infrared fluorescent probe for hydrogen sulfide imaging in vivo was written by Wu, Luyan;Zeng, Wenhui;Feng, Liandong;Hu, Yuxuan;Sun, Yidan;Yan, Yingxiao;Chen, Hong-Yuan;Ye, Deju. And the article was included in Science China: Chemistry in 2020.Formula: C6H5ClO2 The following contents are mentioned in the article:
Ratiometric fluorescent probes hold great promise for in vivo imaging; however, stimuli-activatable ratiometric probes with fluorescence emissions in near-IR (NIR) region are still very few. Herein, we report a hydrogen sulfide (H2S)-activatable ratiometric NIR fluorescent probe (1-SPN) by integrating a H2S-responsive NIR fluorescent probe 1 into a H2S-inert poly[2,6-(4,4-bis-(2-ethylhexyl)-4H-cyclopenta[2,1-b;3,4-b’]dithiophene)-alt-4,7(2,1,3-benzothiadiazole)] (PCPDTBT)-based NIR semiconducting polymer nanoparticle (SPN). 1-SPN shows “always on” PCPDTBT fluorescence at 830 nm and weak probe 1 fluorescence at 725 nm under excitation at 680 nm. The ratio of NIR fluorescence intensities between 725 and 830 is small. Upon interaction with H2S, the fluorescence at 725 nm is rapidly switched on, resulting in a large enhancement of I725/I830, which is allowed for sensitive visualization and quantification of H2S concentrations in living cells. Taking advantage of enhanced tissue penetration depth of NIR fluorescence, 1-SPN is also applied for real-time ratiometric fluorescence imaging of hepatic and tumor H2S in living mice. This study demonstrates that activatable ratiometric NIR fluorescent probes hold great potential for in vivo imaging. This study involved multiple reactions and reactants, such as 4-Chlororesorcinol (cas: 95-88-5Formula: C6H5ClO2).
4-Chlororesorcinol (cas: 95-88-5) belongs to organic chlorides. Organochlorines stimulate the central nervous system and cause convulsions, tremor, nausea, and mental confusion. Examples are dichlorodiphenyltrichloroethane (DDT), chlordane, lindane, endosulfan, and dieldrin. Alkanes and aryl alkanes may be chlorinated under free radical conditions, with UV light. However, the extent of chlorination is difficult to control.Formula: C6H5ClO2
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics