Site-specific covalent labeling of His-tag fused proteins with a reactive Ni(ii)-NTA probe was written by Uchinomiya, Sho-hei;Nonaka, Hiroshi;Fujishima, Sho-hei;Tsukiji, Shinya;Ojida, Akio;Hamachi, Itaru. And the article was included in Chemical Communications (Cambridge, United Kingdom) in 2009.Name: 2-Chloro-5-(chlorosulfonyl)-4-fluorobenzoyl chloride The following contents are mentioned in the article:
A new method for covalent labeling of a His-tag fused protein with a small reactive probe was developed; this method is based on the complementary interaction between the His-tag and Ni(ii)-NTA, which facilitates a nucleophilic reaction between a histidine residue of the tag and the electrophilic tosyl group of the Ni(ii)-NTA probe by the proximity effect. This study involved multiple reactions and reactants, such as 2-Chloro-5-(chlorosulfonyl)-4-fluorobenzoyl chloride (cas: 866763-17-9Name: 2-Chloro-5-(chlorosulfonyl)-4-fluorobenzoyl chloride).
2-Chloro-5-(chlorosulfonyl)-4-fluorobenzoyl chloride (cas: 866763-17-9) belongs to organic chlorides. Organic chlorides can be used in production of: PVC, pesticides, chloromethane, teflon, insulators. Organochlorine compounds are lipophylic, meaning they are more soluble in fat than in water. This gives them a high tenancy to accumulate in the food chain (biomagnification).Name: 2-Chloro-5-(chlorosulfonyl)-4-fluorobenzoyl chloride
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics