Khan, Mohammad Rizwan published the artcileQuantitative determination of methylene blue in environmental samples by solid-phase extraction and ultra-performance liquid chromatography-tandem mass spectrometry: a green approach, COA of Formula: C22H23ClN4, the publication is RSC Advances (2014), 4(64), 34037-34044, database is CAplus.
Industrial effluents with dyes may contain appreciable concentrations of materials with high COD and suspended solids, posing adverse effects to both humans and aquatic life; therefore, these effluents require quant. monitoring. In the present study, an anal. method based on solid-phase extraction (SPE) and ultra-performance liquid chromatog.-tandem mass spectrometry (UPLC-MS/MS) has been optimized for the quant. anal. of methylene blue (MB) in environmental samples. For the extraction of MB, a variety of solvents, including formic acid, were investigated to obtain optimum recovery. MB extraction using the SPE method was best achieved using methanol with 1 M formic acid. Chromatog. separation of MB and methylene violet 3RAX (MV 3RAX, internal standard) was accomplished on an Acquity BEH C18 column using water (64.99%) with formic acid (0.01%) and acetonitrile (35%) in isocratic elution mode. Chromatog. separation for both MB and MV 3RAX was achieved in <2 min with good resolution and superior peak symmetry. MB and MV 3RAX were quantified with electrospray ionization coupled with MS/MS in the multiple reaction monitoring mode. Good quality parameters were achieved for instance linearity (r2 > 0.999), run-to-run and day-to-day precisions with relative standard deviations of <4%, sensitivity with a low limit of detection (LOD) of 0.1 ng mL-1 and limit of quantification (LOQ) of 0.4 ng mL-1 were obtained. Industrial wastewater samples (paper, textile, laundry and printing press) were analyzed, and an MB level was found in between 0.36 and 1.08 μg mL-1 with excellent recovery rates (95-99%) depending on samples.
RSC Advances published new progress about 4569-86-2. 4569-86-2 belongs to chlorides-buliding-blocks, auxiliary class Other Aromatic Heterocyclic,Salt,Amine,Benzene, name is 3-Amino-7-(diethylamino)-5-phenylphenazin-5-ium chloride, and the molecular formula is C22H23ClN4, COA of Formula: C22H23ClN4.
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https://en.wikipedia.org/wiki/Chloride,
Chlorides – an overview | ScienceDirect Topics