Peng, Liang published the artcileSurrogate based accurate quantification of endogenous acetylcholine in murine brain by hydrophilic interaction liquid chromatography-tandem mass spectrometry, Name: 3-Carboxy-N,N,N-trimethylpropan-1-aminium chloride, the publication is Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences (2011), 879(32), 3927-3931, database is CAplus and MEDLINE.
Cholinergic dysfunction is known as a hallmark feature of Alzheimer’s disease (AD). Measurement of endogenous acetylcholine (ACh) in specific brain regions is important in understanding the pathol. of AD and in designing and evaluating novel cholinomimetic agents for the treatment of AD. Since ACh is an endogenous neurotransmitter, there is no real blank matrix available to construct standard curves. It has been a challenging task to determine ACh in complex brain matrixes. To overcome these difficulties, we employed a surrogate analyte strategy using ACh-d4 instead of ACh to generate calibration curves and Ch-d9 as internal standard (IS). The brain samples were deproteinized by acetonitrile with IS. Analytes and IS were separated by a HILIC column with the mobile phase composed of 20 mM ammonium formate in water-acetonitrile (30:70, volume/volume, adjusted to pH 3.0 with formic acid) and monitored in multiple reaction monitoring (MRM) mode using a pos. electrospray source. The concentrations of endogenous ACh were calculated based on the peak area ratio of the analyte to the IS using a regression equation for the corresponding surrogate standard (ACh-d4). The lower limit of detection was 0.2 ng/mL and linearity was maintained over the range of 10-1000 ng/mL. Compared to other currently available methods, this approach offers improved accuracy and precision for efficient anal. of ACh. The proposed method was proved successfully by evaluating the action of typical acetylcholinesterase inhibitor huperzine A in senescence accelerated mouse prone 8 (SAMP8).
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences published new progress about 6249-56-5. 6249-56-5 belongs to chlorides-buliding-blocks, auxiliary class Phase Transfer Catalyst,Inhibitor,Natural product, name is 3-Carboxy-N,N,N-trimethylpropan-1-aminium chloride, and the molecular formula is C7H16ClNO2, Name: 3-Carboxy-N,N,N-trimethylpropan-1-aminium chloride.
Referemce:
https://en.wikipedia.org/wiki/Chloride,
Chlorides – an overview | ScienceDirect Topics