In-situ growth of nitrogen-doped carbonized polymer dots on black phosphorus for electrochemical DNA biosensor of Escherichia coli O157: H7 was written by Shi, Fan;Wang, Baoli;Yan, Lijun;Wang, Bei;Niu, Yanyan;Wang, Lisi;Sun, Wei. And the article was included in Bioelectrochemistry in 2022.Electric Literature of C16H18ClN3S This article mentions the following:
Sensitive and accurate detection technol. for pathogenic bacteria is of great social and economic significance in foodborne disease and food safety. In this paper, a novel portable electrochem. DNA biosensor for the detection of specific DNA sequence of Escherichia coli (E. coli) O157: H7 was constructed. To enhance the performance of the electrochem. sensor, a functionalized nitrogen-doped carbonized polymer dots in-situ grown on few-layer black phosphorus (N-CPDs@FLBP) was synthesized and used as the modifier on the surface of screen-printed electrode. Combining gold nanoparticles as immobilization matrix and methylene blue as electrochem. indicator, the anal. performance of this electrochem. DNA biosensor was evaluated using standard complementary ssDNA sequence in the linear concentration range from 1.0 x 10-19 to 1.0 x 10-6 mol/L with a low detection limit as 3.33 x 10-20 mol/L (3 σ). Furthermore, the portable electrochem. DNA biosensor was proposed based on polymerase chain reaction amplification for the detection of the E. coli O157: H7 genomic DNA from chicken meat, which verified the feasibility for practical samples detection. The research has great theor. and practical significance for the development of electrochem. biosensor of pathogenic bacteria. In the experiment, the researchers used many compounds, for example, 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride (cas: 61-73-4Electric Literature of C16H18ClN3S).
3,7-Bis(dimethylamino)phenothiazin-5-ium chloride (cas: 61-73-4) belongs to organic chlorides. An organic chloride is an organic compound containing at least one covalently bonded atom of chlorine. Their wide structural variety and divergent chemical properties lead to a broad range of names and applications. Organochlorine compounds are lipophylic, meaning they are more soluble in fat than in water. This gives them a high tenancy to accumulate in the food chain (biomagnification).Electric Literature of C16H18ClN3S
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics