SkinEthic HCE Time-to-Toxicity on solids: A test method for distinguishing chemicals inducing serious eye damage, eye irritation and not requiring classification and labeling was written by Alepee, Nathalie;Leblanc, Virginie;Grandidier, Marie-Helene;Teluob, Severine;Viricel, Anaelle;Adriaens, Els;Michaut, Valerie. And the article was included in Toxicology In Vitro in 2021.Application of 101-20-2 This article mentions the following:
This study describes the development of a Time-to-Toxicity approach for solids (TTS) based on the SkinEthic HCE tissue construct, capable to distinguish chems. that do not require classification for serious eye damage/eye irritation (No Cat.) from chems. that require classification for eye irritation (Cat. 2), and serious eye damage (Cat. 1). Briefly, the time-to-toxicity of 69 solids was evaluated by exposing SkinEthic HCE tissue constructs to the test chem. for two different time periods (30-min, and 120-min). Based on the viability observed for the different exposure periods, a classification was assigned. The within laboratory reproducibility in terms of concordance in classifications (3 UN GHS categories), based on a set of 48 solids, was 93.7%. Furthermore, 73.6% Cat. 1 (N = 24), 55.6% Cat. 2 (N = 15) and 72.2% No Cat. (N = 30) were correctly identified with the SkinEthic HCE TTS test method. This study provides evidence that the SkinEthic HCE Time-to-Toxicity method (multiple exposure times) can distinguish Cat. 2 solids from Cat. 1 solids. This is an added value compared to the SkinEthic HCE EITS method (single exposure time) that can distinguish No Cat. chems. from chems. that do require classification and labeling for eye irritation/serious eye damage (Cat. 2/Cat. 1). In the experiment, the researchers used many compounds, for example, 1-(4-Chlorophenyl)-3-(3,4-dichlorophenyl)urea (cas: 101-20-2Application of 101-20-2).
1-(4-Chlorophenyl)-3-(3,4-dichlorophenyl)urea (cas: 101-20-2) belongs to organic chlorides. Organic chlorides are compounds containing a carbon-chlorine bond, which are widely used in the oil field as a wax dissolver. Organochlorine compounds are lipophylic, meaning they are more soluble in fat than in water. This gives them a high tenancy to accumulate in the food chain (biomagnification).Application of 101-20-2
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics