Synthesis and Pharmacophore Modeling of 2,6,9-Trisubstituted Purine Derivatives and Their Potential Role as Apoptosis-Inducing Agents in Cancer Cell Lines was written by Calderon-Arancibia, Jeannette;Espinosa-Bustos, Christian;Canete-Molina, Alvaro;Tapia, Ricardo A.;Faundez, Mario;Torres, Maria Jose;Aguirre, Adam;Paulino, Margot;Salas, Cristian O.. And the article was included in Molecules in 2015.Application of 203436-45-7 This article mentions the following:
A series of 2,6,9-trisubstituted purine derivatives I (R1 = i-Pr, Bu, i-Bu, i-Pe, Hx, Pe; R2 = H, OMe) have been synthesized and investigated for their potential role as antitumor agents. Twelve compounds were obtained by a three step synthetic procedure using microwave irradiation in a pivotal step. All the synthesized compounds were evaluated in vitro to determine their potential effect on cell toxicity by the MTT method and flow cytometry anal. on four cancer cells lines and Vero cells. Three out of twelve compounds were found to be promising agents compared to a known and effective anticancer drug, etoposide, in three out of four cancer cell lines assayed with considerable selectivity. The preliminary flow cytometry data suggests that compounds mentioned above induce apoptosis on these cells. The main structural requirements for their activity for each cancer cell line were characterized with a preliminary pharmacophore model, which identified aromatic centers, hydrogen acceptor/donor center, and a hydrophobic area. These features were consistent with the cytotoxic activity of the assayed compounds In the experiment, the researchers used many compounds, for example, 2,6-Dichloro-9-isopropyl-9H-purine (cas: 203436-45-7Application of 203436-45-7).
2,6-Dichloro-9-isopropyl-9H-purine (cas: 203436-45-7) belongs to organic chlorides. Chlorinated organic compounds are found in nearly every class of biomolecules and natural products including alkaloids, terpenes, amino acids, flavonoids, steroids, and fatty acids. Alkyl chlorides are versatile building blocks in organic chemistry. While alkyl bromides and iodides are more reactive, alkyl chlorides tend to be less expensive and more readily available.Application of 203436-45-7
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics