Investigation of pyrazolo[1,5-a]quinoxalin-4-ones as novel monoamine oxidase inhibitors was written by Panova, Valeria A.;Filimonov, Sergey I.;Chirkova, Zhanna V.;Kabanova, Mariya V.;Shetnev, Anton A.;Korsakov, Mikhail K.;Petzer, Anel;Petzer, Jacobus P.;Suponitsky, Kyrill Yu. And the article was included in Bioorganic Chemistry in 2021.Synthetic Route of C9H8ClNO4 This article mentions the following:
The present study synthesized a series of pyrazolo[1,5-a]quinoxalin-4-one derivatives I [R1 = H, Me, MeO, Cl; R2 = NH2, CN, Me(CO)NH, Et(O)OC; R3 = H, HO, acetyloxidanyl, (2-ethoxy-2-oxoethyl)oxidanyl] and evaluated them as potential inhibitors of human MAO-A and MAO-B. The results showed that derivatives I [R1 = H, MeO, Cl; R2 = NH2, Me(CO)NH; R3 = H, HO, acetyloxidanyl] inhibited MAO-A, and derivatives I [R1 = Me, MeO; R2 = CN; R3 = H, (2-ethoxy-2-oxoethyl)oxidanyl] inhibited MAO-B with IC50 values in the submicromolar range (<1μM). The most potent MAO-A inhibitor, I [R1 = Cl, R2 = MeC(O)NH, R3 = acetyloxidanyl], exhibited an IC50 value of 0.028μM and displayed 50-fold selectivity for MAO-A over MAO-B. The most potent MAO-B inhibitor, I [R1 = Me, R2 = CN, R3 = H], exhibited an IC50 value of 0.617μM and displayed 8-fold selectivity for MAO-B. This was the first reported MAO inhibition by pyrazolo[1,5-a]quinoxalin-4-one derivatives, I and this study concluded that these compounds I were suitable lead for the future development of MAO inhibitors, particularly of the MAO-A isoform. In the experiment, the researchers used many compounds, for example, Ethyl 4-chloro-3-nitrobenzoate (cas: 16588-16-2Synthetic Route of C9H8ClNO4).
Ethyl 4-chloro-3-nitrobenzoate (cas: 16588-16-2) belongs to organic chlorides. Organic chlorides can be used in production of: PVC, pesticides, chloromethane, teflon, insulators. Alkyl chlorides readily react with amines to give substituted amines. Alkyl chlorides are substituted by softer halides such as the iodide in the Finkelstein reaction.Synthetic Route of C9H8ClNO4
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics