Validation of the SkinEthic HCE Time-to-Toxicity test method for eye hazard classification of chemicals according to UN GHS was written by Alepee, N.;Grandidier, M. H.;Teluob, S.;Amaral, F.;Caviola, E.;De Servi, B.;Martin, S.;Meloni, M.;Nardelli, L.;Pasdelou, C.;Tagliati, V.;Viricel, A.;Adriaens, E.;Michaut, V.. And the article was included in Toxicology In Vitro in 2022.Application of 96568-04-6 This article mentions the following:
This study describes the within- and between-laboratory reproducibility (WLR and BLR) of a Time-to-Toxicity (TTT) approach for chems. based on the SkinEthic HCE tissue construct, capable to distinguish chems. that do not require classification for serious eye damage/eye irritation (No Cat.) from chems. that require classification for eye irritation (Cat. 2), and serious eye damage (Cat. 1). The WLR and BLR was assessed with three participating laboratories Each laboratory tested 40 coded chems. in three independent runs. The predictive capacity of the method was assessed on a larger set of 150 chems. (70 liquids and 80 solids) by combining the results of this study with the results of the test method developer. The WLR for the 20 liquids ranged from 85% to 95% with a BLR of 90%. For the 20 solids, a WLR and BLR of 100% was obtained. The test method developer obtained a WLR of 80% and 95%, based on 50 liquids and 48 solids tested in three independent runs, resp. Regarding the predictive capacity, the SkinEthic HCE TTT test method identified 80.8% Cat. 1, 69.2% Cat. 2, and 74.9% No Cat. correctly. An independent peer review panel concluded that based on all available data, the relevance and reliability of the SkinEthic HCE TTT has been demonstrated for discriminating the three UN GHS eye hazard categories. In the experiment, the researchers used many compounds, for example, Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate (cas: 96568-04-6Application of 96568-04-6).
Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate (cas: 96568-04-6) belongs to organic chlorides. Organic chlorides can cause corrosion in pipelines, valves and condensers, and cause catalyst poisoning. The hydrocarbon processing industry (HPI) and others are affected by damage caused by these substances. Alkyl chlorides are versatile building blocks in organic chemistry. While alkyl bromides and iodides are more reactive, alkyl chlorides tend to be less expensive and more readily available.Application of 96568-04-6
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics