A high-throughput screening assay for identification of chemicals with liver tumor promoting potential using a transgenic zebrafish line was written by Chen, Sheng;Wu, Jian;Li, Meng;Sun, Qian;Gong, Zhiyuan;Letcher, Robert J.;Liu, Chunsheng. And the article was included in Chemosphere in 2022.Quality Control of 1-(4-Chlorophenyl)-3-(3,4-dichlorophenyl)urea This article mentions the following:
Traditional high-throughput methods for identification of chems. with liver tumor promotion potentials are based on established cancer cell lines, and rapid and cost-effective high-throughput screening assays in whole organisms are presently lacking. In this study, a transgenic zebrafish liver cancer model was employed to develop a method that could be used to identify chems. with liver tumor promotion effect quickly and accurately. The method consisted of three parts, including exposure preparation, exposure process and image acquisition. In brief, after chem. exposure for 7 days, 96-well plate exposure system for zebrafish larvae was assessed by microplate reader. Then, the liver cancer promoting potential chems. were evaluated by field area and field average intensity of fluorescence. The results were further validated by conducting histopathol. examination Our data demonstrated that the high-throughput screening assay developed in this study was reproducible and could be used to rapidly screen chems. with liver tumor promoting potentials by using tris-(2-chloropropyl)-phosphate (TDCIPP) as a pos. control. Furthermore, some other pos. chems. found in previous studies and environmental compounds were assessed using the established method. Results indicated that 86.7% of the pos. chems. and five environmental compounds out of seventeen compounds could enhance liver tumor progression. In the experiment, the researchers used many compounds, for example, 1-(4-Chlorophenyl)-3-(3,4-dichlorophenyl)urea (cas: 101-20-2Quality Control of 1-(4-Chlorophenyl)-3-(3,4-dichlorophenyl)urea).
1-(4-Chlorophenyl)-3-(3,4-dichlorophenyl)urea (cas: 101-20-2) belongs to organic chlorides. Organochlorines are organic compounds having multiple chlorine atoms. They were the first synthetic pesticides that were used in agriculture. They are resistant to most microbial and chemical degradations.While alkyl bromides and iodides are more reactive, alkyl chlorides tend to be less expensive and more readily available. Alkyl chlorides readily undergo attack by nucleophiles.Quality Control of 1-(4-Chlorophenyl)-3-(3,4-dichlorophenyl)urea
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics