Misik, Miroslav; Nersesyan, Armen; Kment, Michael; Ernst, Benjamin; Setayesh, Tahereh; Ferk, Franziska; Holzmann, Klaus; Krupitza, Georg; Knasmueller, Siegfried published the artcile< Micronucleus assays with the human derived liver cell line (Huh6): A promising approach to reduce the use of laboratory animals in genetic toxicology>, Reference of 6055-19-2, the main research area is micronucleus human liver cell line genetic toxicol; Cytokinesis-block micronucleus assay; Genotoxicity; Huh6; In vitro assay; Micronucleus; Sensitivity; Specificity.
The inadequate representation of enzymes which catalyze the activation/detoxification of xenobiotics in cells that are currently used in genotoxicity testing of chems. leads to a high number of false pos. results and the number of follow up studies with rodents could be reduced by use of more reliable in vitro models. We found earlier that several xenobiotic drug metabolizing enzymes are represented in the human derived liver cell line Huh6 and developed a protocol for micronucleus (MN) experiments which is in agreement with the current OECD guideline. This protocol was used to test 23 genotoxic and non-genotoxic reference chems.; based on these results and of earlier findings (with 9 chems.) we calculated the predictive value of the assay for the detection of genotoxic carcinogens. We found a sensitivity of 80% and a specificity of 94% for a total number of 32 chems.; comparisons with results obtained with other in vitro assays show that the validity of MN tests with Huh6 is higher as that of other exptl. models. These results are promising and indicate that the use of Huh6 cells in genetic toxicol. may contribute to the reduction of the use of laboratory rodents; further exptl. work to confirm this assumption is warranted.
Food and Chemical Toxicology published new progress about Cytokinesis. 6055-19-2 belongs to class chlorides-buliding-blocks, and the molecular formula is C7H17Cl2N2O3P, Reference of 6055-19-2.
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