Kim, Sou Hyun; Kim, Minwoo; Kwon, Doyoung; Pyo, Jae Sung; Kim, Joo Hyun; Kwak, Jae-Hwan; Jung, Young-Suk published the artcile< N-phenyl cinnamamide derivatives protect hepatocytes against oxidative stress by inducing cellular glutathione synthesis via nuclear factor (erythroid-derived 2)-like 2 activation>, HPLC of Formula: 17082-09-6, the main research area is phenyl cinnamamide preparation antioxidant hepatocyte Nrf2 ARE agonistic activity; N-phenyl cinnamamide; antioxidant response element; glutathione; nuclear factor (erythroid-derived 2)-like 2.
Substituted N-Ph cinnamamide derivatives I (R = OMe, OEt, CN, Cl, N(Me)2) were designed and synthesized to confirm activation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) pathway by the electronic effect on beta-position of Michael acceptor according to introducing the R1 and R2 group. Compounds were screened using the Nrf2/antioxidant response element (ARE)-driven luciferase reporter assay. Compound II (R = N(Me)2) showed desirable luciferase activity in HepG2 cells without cell toxicity. MRNA and protein expression of Nrf2/ARE target genes such as NAD(P)H quinone oxidoreductase 1, hemeoxygenase-1, and glutamate-cysteine ligase catalytic subunit (GCLC) were upregulated by compound II (R = N(Me)2) in a concentration-dependent manner. Treatment with compound II (R = N(Me)2) resulted in increased endogenous antioxidant glutathione, showing strong correlation with enhanced GCLC expression for synthesis of glutathione. In addition, tert-Bu hydroperoxide (t-BHP)-generated reactive oxygen species were significantly removed by compound II (R = N(Me)2) , and the results of a cell survival assay in a t-BHP-induced oxidative cell injury model showed a cytoprotective effect of compound II (R = N(Me)2) in a concentration dependent manner. In conclusion, the novel compound II (R = N(Me)2) can be utilized as an Nrf2/ARE activator in antioxidative therapy.
Molecules published new progress about Antioxidants. 17082-09-6 belongs to class chlorides-buliding-blocks, and the molecular formula is C9H7ClO, HPLC of Formula: 17082-09-6.
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics