Imaging Ca2+ with a Fluorescent Rhodol was written by Contractor, Alisha A.;Miller, Evan W.. And the article was included in Biochemistry in 2018.Related Products of 95-88-5 The following contents are mentioned in the article:
Ca2+ mediates a host of biochem. and biophys. signaling processes in cells. The development of synthetic, Ca2+-sensitive fluorophores has played an instrumental role in the authors’ understanding of the temporal and spatial dynamics of Ca2+. Coupling Ca2+-selective ligands to fluorescent reporters provided a wealth of excellent indicators that span the visible excitation and emission spectrum and possess Ca2+ affinities suited to a variety of cellular contexts. One underdeveloped area is the use of hybrid rhodamine/fluorescein fluorophores, or rhodols, in the context of Ca2+ sensing. Rhodols are bright and photostable and have good two-photon absorption cross sections (σTPA), making them excellent candidates for incorporation into Ca2+-sensing scaffolds. Here, the authors present the design, synthesis, and application of rhodol Ca2+ sensor 1 (RCS-1) (I), a chlorinated pyrrolidine-based rhodol. RCS-1 possesses a Ca2+ binding constant of 240 nM and a 10-fold turn response to Ca2+. RCS-1 effectively absorbs IR light and has a σTPA of 76 GM at 840 nm, 3-fold greater than that of its fluorescein-based counterpart. The acetoxy-Me ester of I stains the cytosol of live cells, enabling observation of Ca2+ fluctuations and cultured neurons using both one- and two-photon illumination. Together, these results demonstrate the utility of rhodol-based scaffolds for Ca2+ sensing using two-photon illumination in neurons. This study involved multiple reactions and reactants, such as 4-Chlororesorcinol (cas: 95-88-5Related Products of 95-88-5).
4-Chlororesorcinol (cas: 95-88-5) belongs to organic chlorides. Organic chlorides can be used in production of: PVC, pesticides, chloromethane, teflon, insulators. Organochlorine compounds are lipophylic, meaning they are more soluble in fat than in water. This gives them a high tenancy to accumulate in the food chain (biomagnification).Related Products of 95-88-5
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics