A near infrared fluorescent probe for the detection and imaging of prolyl aminopeptidase activity in living cells was written by Liu, Xianjun;Zhang, Quan-Yu;Wang, Fenglin;Jiang, Jian-Hui. And the article was included in Analyst (Cambridge, United Kingdom) in 2019.Computed Properties of C6H5ClO2 The following contents are mentioned in the article:
Prolyl aminopeptidase (PAP) is an important exopeptidase which might be a biomarker for pathogen infection and a potential therapeutic target. However, very few fluorescent probes have been developed for detecting PAP activity. Here we report the development of the first near IR (NIR) turn-on fluorescent probe (NIR-PAP) for detecting and imaging PAP in living cells. The probe is prepared by reacting a cysteine-proline dipeptide with an acryloylated NIR fluorophore via a facile thiol-Michael addition reaction. NIR-PAP exhibits a dynamic response toward PAP in the range of 0.02-2.5 U mL-1 with an estimated limit of detection of 0.013 U mL-1. In vitro studies also reveal that the probe displays high specificity and robust responses toward PAP under physiol. pH and temperature conditions. Moreover, NIR-PAP is successfully introduced to detect and differentiate PAP activity in four different cell lines via both confocal fluorescence imaging and flow cytometry. Therefore, our probe may hold great promise in diagnosing infectious diseases caused by pathogens and screening therapeutic drugs in vivo. This study involved multiple reactions and reactants, such as 4-Chlororesorcinol (cas: 95-88-5Computed Properties of C6H5ClO2).
4-Chlororesorcinol (cas: 95-88-5) belongs to organic chlorides. Organic chlorides can be used in production of: PVC, pesticides, chloromethane, teflon, insulators. Alkanes and aryl alkanes may be chlorinated under free radical conditions, with UV light. However, the extent of chlorination is difficult to control.Computed Properties of C6H5ClO2
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics