Ogris, Manfred published the artcileNovel biocompatible cationic copolymers based on polyaspartylhydrazide being potent as gene vector on tumor cells, COA of Formula: C7H16ClNO2, the publication is Pharmaceutical Research (2007), 24(12), 2213-2222, database is CAplus and MEDLINE.
The reaction between α,β-poly(aspartylhydrazide) (PAHy), a water soluble synthetic polymer and 3-(carboxypropyl)trimethyl-ammonium chloride (CPTACl) produced copolymers bearing permanent pos. charges (PAHy-CPTA) with mol. weight of 10 kDa and PAHy-CPTA copolymers differing in pos. charge amount (18-58%) were chosen for biol. investigations. Biophys. properties of DNA/PAHy-CPTA polyplexes were evaluated in terms of DNA condensation, zeta potential and size distribution. Cytotoxicity studies on Neuro2A murine neuroblastoma cells evidenced absence of toxicity of these copolymers up to 300 μg/mL unlike linear polyethylenimine (LPEI) that was highly toxic already at 20 μg/mL. PAHy-CPTA copolymers did not induce any erythrocyte aggregation up to 1 mg/mL. Cellular interaction studies of PAHy-CPTA polyplexes evidenced a faster binding of these polyplexes with cells compared to DNA/LPEI polyplexes. The in vitro transfection ability of PAHy-CPTA polyplexes was strongly affected by exptl. conditions reaching about 10% of the transfection efficiency of optimized LPEI polyplexes. Finally, in vivo application studies confirmed the biocompatibility of PAHy-CPTA copolymers. With LPEI, clear signs of microvesicular fatty liver were observed and with LPEI polyplexes significant weight loss. In strong contrast, PAHy-CPTA did not induce histopathol. changes or weight loss.
Pharmaceutical Research published new progress about 6249-56-5. 6249-56-5 belongs to chlorides-buliding-blocks, auxiliary class Phase Transfer Catalyst,Inhibitor,Natural product, name is 3-Carboxy-N,N,N-trimethylpropan-1-aminium chloride, and the molecular formula is C7H16ClNO2, COA of Formula: C7H16ClNO2.
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https://en.wikipedia.org/wiki/Chloride,
Chlorides – an overview | ScienceDirect Topics