Balaraman, Harish Babu published the artcileHigh selective purification of IgY from quail egg: Process design and quantification of deep eutectic solvent based ultrasound assisted liquid phase microextraction coupled with preparative chromatography, HPLC of Formula: 23616-79-7, the publication is International Journal of Biological Macromolecules (2020), 253-262, database is CAplus and MEDLINE.
The present research investigates on task-specific deep eutectic solvents (TDES) based aqueous two-phase extraction and purification of Igs by chromatog. from quail egg. The synthesis of TDES was accomplished with quaternary ammonium salt as hydrogen bond acceptor (HBA) and glycerol as hydrogen bond donor (HBD). Aqueous two-phase (ATPS) formation of TDESs with various salts was established and phase partitioning ability was evaluated using standard bovine Ig. Ultrasound-assisted liquid-liquid microextraction (UA-LLME) was performed with ATPS of better partitioning ability for quail egg yolk. Optimization of influential variables with response surface methodol. was accomplished and maximum yield was achieved for 85% (volume/volume) of NADES for feed of 18μg/mL of egg yolk isolate with ultrasound temperature of 35°C and 12 min contact time resp. The quantification of recovery was accomplished by gel filtration process with the determination of retention time, volume and resolution of separation using marker protein. Ultrapure Ig-Y (IgY) is achieved with anion exchange chromatog. and yield was calculated The results revealed that the selective concentration of Ig-Y from quail egg yolk could be performed using environment-friendly TDES based UA-LLME coupled with chromatog.
International Journal of Biological Macromolecules published new progress about 23616-79-7. 23616-79-7 belongs to chlorides-buliding-blocks, auxiliary class Phase Transfer Catalyst, name is N-Benzyl-N,N-dibutylbutan-1-aminium chloride, and the molecular formula is C19H34ClN, HPLC of Formula: 23616-79-7.
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