A novel conductive nanocomposite-based biosensor for ultrasensitive detection of microRNA-21 in serum, using methylene blue as mediator was written by Deng, Mengtian;Feng, Jialu;Tao, Dan;Yan, Hanhui;Ding, Jingjing;Jaffrezic-Renault, Nicole;Guo, Zhenzhong. And the article was included in Bioelectrochemistry in 2022.Quality Control of 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride This article mentions the following:
MicroRNA-21 (miRNA-21) is a common biomarker with high expression in breast tumors. Therefore, sensitive detection of miRNA-21 is of great significance for clin. breast tumor diagnosis. A TH/rGO/CMK-3/AuNPs nanocomposite is composed of thionine (TH), reduced graphene oxide (rGO), ordered mesoporous carbon (CMK-3), and gold nanoparticles (AuNPs), which help to increase the sp. surface area of a glassy carbon electrode (GCE) and to amplify the DPV signal. Meanwhile, methylene blue (MB) was combined with the capture probe guanine and absorbed by the composite material to mediate the differential pulse voltammetry (DPV) of the obtained miRNA biosensor. The current response decreased with increasing miRNA-21 concentration under optimal conditions. The biosensor responds to miRNA-21 in the 0.1fM-1 pM concentration range, and the detection limit (LOD) was 0.046 fM. Moreover, human serum samples were effectively detected utilizing the miRNA-21 biosensor with satisfactory results. In the experiment, the researchers used many compounds, for example, 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride (cas: 61-73-4Quality Control of 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride).
3,7-Bis(dimethylamino)phenothiazin-5-ium chloride (cas: 61-73-4) belongs to organic chlorides. An organic chloride is an organic compound containing at least one covalently bonded atom of chlorine. Their wide structural variety and divergent chemical properties lead to a broad range of names and applications. Alkyl chlorides readily react with amines to give substituted amines. Alkyl chlorides are substituted by softer halides such as the iodide in the Finkelstein reaction.Quality Control of 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride
Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics