Category: 96568-04-6

Reprint of “CON4EI: Bovine Corneal Opacity and Permeability (BCOP) test for hazard identification and labelling of eye irritating chemicals” was written by Verstraelen, Sandra;Maglennon, Gareth;Hollanders, Karen;Boonen, Francis;Adriaens, Els;Alepee, Nathalie;Drzewiecka, Agnieszka;Gruszka, Katarzyna;Kandarova, Helena;Willoughby, Jamin A. Sr.;Guest, Robert;Schofield, Jane;Van Rompay, An R.. And the article was included in Toxicology In Vitro in 2018.COA of Formula: C10H8Cl2FNO3 This article mentions the following:

Assessment of ocular irritation potential is an international regulatory requirement in the safety evaluation of industrial and consumer products. None in vitro ocular irritation assays are capable of fully categorizing chems. as stand-alone. Therefore, the CEFIC-LRI-AIMT6-VITO CON4EI consortium assessed the reliability of eight in vitro test methods and computational models as well as established a tiered-testing strategy. One of the selected assays was Bovine Corneal Opacity and Permeability (BCOP). In this project, the same corneas were used for measurement of opacity using the OP-KIT, the Laser Light-Based Opacitometer (LLBO) and for histopathol. anal. The results show that the accuracy of the BCOP OP-KIT in identifying Cat 1 chems. was 73.8% while the accuracy was 86.3% for No Cat chems. BCOP OP-KIT false neg. were often related to an in vivo classification driven by conjunctival effects only. For the BCOP LLBO, the accuracy in identifying Cat 1 chems. was 74.4% vs. 88.8% for No Cat chems. The BCOP LLBO seems very promising for the identification of No Cat liquids but less so for the identification of solids. Histopathol. as an addnl. endpoint to the BCOP test method does not reduce the false neg. rate substantially for in vivo Cat 1 chems. In the experiment, the researchers used many compounds, for example, Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate (cas: 96568-04-6COA of Formula: C10H8Cl2FNO3).

Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate (cas: 96568-04-6) belongs to organic chlorides. Chlorinated organic compounds are found in nearly every class of biomolecules and natural products including alkaloids, terpenes, amino acids, flavonoids, steroids, and fatty acids.While alkyl bromides and iodides are more reactive, alkyl chlorides tend to be less expensive and more readily available. Alkyl chlorides readily undergo attack by nucleophiles.COA of Formula: C10H8Cl2FNO3

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Development of the EpiOcular eye irritation test for hazard identification and labelling of eye irritating chemicals in response to the requirements of the EU cosmetics directive and REACH legislation was written by Kaluzhny, Yulia;Kandarova, Helena;Hayden, Patrick;Kubilus, Joseph;d’Argembeau-Thornton, Laurence;Klausner, Mitchell. And the article was included in ATLA, Alternatives to Laboratory Animals in 2011.Quality Control of Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate This article mentions the following:

The recently implemented 7th Amendment to the EU Cosmetics Directive and the EU REACH legislation have heightened the need for in vitro ocular test methods. To address this need, the EpiOcular eye irritation test (EpiOcular-EIT), which utilizes the normal (non-transformed) human cell-based EpiOcular tissue model, has been developed. The EpiOcular-EIT prediction model is based on an initial training set of 39 liquid and 21 solid test substances and uses a single exposure period and a single cut-off in tissue viability, as determined by the MTT assay. A chem. is classified as an irritant (GHS Category 1 or 2), if the tissue viability is 鈮?60%, and as a non-irritant (GHS unclassified), if the viability is > 60%. EpiOcular-EIT results for the training set, along with results for an addnl. 52 substances, which included a range of alcs., hydrocarbons, amines, esters, and ketones, discriminated between ocular irritants and non-irritants with 98.1% sensitivity, 72.9% specificity, and 84.8% accuracy. To ensure the long-term com. viability of the assay, EpiOcular tissues produced by using three alternative cell culture inserts were evaluated in the EpiOcular-EIT with 94 chems. The assay results obtained with the initial insert and the three alternative inserts were very similar, as judged by correlation coefficients (r²) that ranged from 0.82 to 0.96. The EpiOcular-EIT was pre-validated in 2007/2008, and is currently involved in a formal, multi-laboratory validation study sponsored by the European Cosmetics Association (COLIPA) under the auspices of the European Center for the Validation of Alternative Methods (ECVAM). The EpiOcular-EIT, together with EpiOcular’s long history of reproducibility and proven utility for ultra-mildness testing, make EpiOcular a useful model for addressing current legislation related to animal use in the testing of potential ocular irritants. In the experiment, the researchers used many compounds, for example, Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate (cas: 96568-04-6Quality Control of Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate).

Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate (cas: 96568-04-6) belongs to organic chlorides. Organochlorines stimulate the central nervous system and cause convulsions, tremor, nausea, and mental confusion. Examples are dichlorodiphenyltrichloroethane (DDT), chlordane, lindane, endosulfan, and dieldrin. Aryl chlorides may be prepared by the Friedel-Crafts halogenation, using chlorine and a Lewis acid catalyst.Quality Control of Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Validation of the SkinEthic HCE Time-to-Toxicity test method for eye hazard classification of chemicals according to UN GHS was written by Alepee, N.;Grandidier, M. H.;Teluob, S.;Amaral, F.;Caviola, E.;De Servi, B.;Martin, S.;Meloni, M.;Nardelli, L.;Pasdelou, C.;Tagliati, V.;Viricel, A.;Adriaens, E.;Michaut, V.. And the article was included in Toxicology In Vitro in 2022.Application of 96568-04-6 This article mentions the following:

This study describes the within- and between-laboratory reproducibility (WLR and BLR) of a Time-to-Toxicity (TTT) approach for chems. based on the SkinEthic HCE tissue construct, capable to distinguish chems. that do not require classification for serious eye damage/eye irritation (No Cat.) from chems. that require classification for eye irritation (Cat. 2), and serious eye damage (Cat. 1). The WLR and BLR was assessed with three participating laboratories Each laboratory tested 40 coded chems. in three independent runs. The predictive capacity of the method was assessed on a larger set of 150 chems. (70 liquids and 80 solids) by combining the results of this study with the results of the test method developer. The WLR for the 20 liquids ranged from 85% to 95% with a BLR of 90%. For the 20 solids, a WLR and BLR of 100% was obtained. The test method developer obtained a WLR of 80% and 95%, based on 50 liquids and 48 solids tested in three independent runs, resp. Regarding the predictive capacity, the SkinEthic HCE TTT test method identified 80.8% Cat. 1, 69.2% Cat. 2, and 74.9% No Cat. correctly. An independent peer review panel concluded that based on all available data, the relevance and reliability of the SkinEthic HCE TTT has been demonstrated for discriminating the three UN GHS eye hazard categories. In the experiment, the researchers used many compounds, for example, Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate (cas: 96568-04-6Application of 96568-04-6).

Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate (cas: 96568-04-6) belongs to organic chlorides. Organic chlorides can cause corrosion in pipelines, valves and condensers, and cause catalyst poisoning. The hydrocarbon processing industry (HPI) and others are affected by damage caused by these substances. Alkyl chlorides are versatile building blocks in organic chemistry. While alkyl bromides and iodides are more reactive, alkyl chlorides tend to be less expensive and more readily available.Application of 96568-04-6

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Synthesis of tosufloxacin p-tosylate was written by Liu, Mingliang;Sun, Lanying;Wei, Yonggang;Guo, Huiyuan. And the article was included in Zhongguo Yiyao Gongye Zazhi in 2003.Safety of Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate This article mentions the following:

The title compound was prepared from Et 2,6-dichloro-5-fluoronicotinoylacetate via condensation with CH(OEt)₃, 2,4-difluoroaniline displacement, cyclization, condensation with 3-aminopyrrolidine and hydrolysis in overall yield 72.6%. In the experiment, the researchers used many compounds, for example, Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate (cas: 96568-04-6Safety of Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate).

Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate (cas: 96568-04-6) belongs to organic chlorides. Organochlorines are organic compounds having multiple chlorine atoms. They were the first synthetic pesticides that were used in agriculture. They are resistant to most microbial and chemical degradations. Alkanes and aryl alkanes may be chlorinated under free radical conditions, with UV light. However, the extent of chlorination is difficult to control.Safety of Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Synthesis of tosufloxacin p-tosylate was written by Liu, Mingliang;Sun, Lanying;Wei, Yonggang;Guo, Huiyuan. And the article was included in Zhongguo Yiyao Gongye Zazhi in 2003.Safety of Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate This article mentions the following:

The title compound was prepared from Et 2,6-dichloro-5-fluoronicotinoylacetate via condensation with CH(OEt)₃, 2,4-difluoroaniline displacement, cyclization, condensation with 3-aminopyrrolidine and hydrolysis in overall yield 72.6%. In the experiment, the researchers used many compounds, for example, Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate (cas: 96568-04-6Safety of Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate).

Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate (cas: 96568-04-6) belongs to organic chlorides. Organochlorines are organic compounds having multiple chlorine atoms. They were the first synthetic pesticides that were used in agriculture. They are resistant to most microbial and chemical degradations. Alkanes and aryl alkanes may be chlorinated under free radical conditions, with UV light. However, the extent of chlorination is difficult to control.Safety of Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics

Anticancer and immunomodulatory activities of novel 1,8-naphthyridine derivatives was written by Kumar, Vivek;Madaan, Alka;Sanna, Vinod K.;Vishnoi, Manupriya;Joshi, Narendra;Singh, Anu T.;Jaggi, Manu;Sharma, Pramod K.;Irchhaiya, Raghuveer;Burman, Anand C.. And the article was included in Journal of Enzyme Inhibition and Medicinal Chemistry in 2009.Name: Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate This article mentions the following:

A number of 1,8-naphthyridine derivatives (22-62) have been synthesized and screened for their in vitro cytotoxicity against eight tumors and two non-tumor cell lines. Halogen substituted 1,8-naphthyridine-3-caboxamide derivatives showed potent activity with compound 47 (I) having IC₅₀ of 0.41 and 0.77 μM on MIAPaCa and K-562 cancer cell lines, resp. while, compound 36 had IC₅₀ of 1.19 μM on PA-1 cancer cell line. However, one of the unsubstituted 1,8-naphthyridine-C-3′-heteroaryl derivative 29 showed potent cytotoxicity with IC₅₀ of 0.41 and 1.4 μM on PA-1 and SW620 cancer cell lines, resp. These compounds were also evaluated for anti-inflammatory activity as suggested by downregulation of proinflammatory cytokines. In the experiment, the researchers used many compounds, for example, Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate (cas: 96568-04-6Name: Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate).

Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate (cas: 96568-04-6) belongs to organic chlorides. Organochlorines are organic compounds having multiple chlorine atoms. They were the first synthetic pesticides that were used in agriculture. They are resistant to most microbial and chemical degradations. Organochlorine compounds are lipophylic, meaning they are more soluble in fat than in water. This gives them a high tenancy to accumulate in the food chain (biomagnification).Name: Ethyl 3-(2,6-Dichloro-5-fluoro-3-pyridyl)-3-oxopropionate

Referemce:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics