Zhang, Chen;Tian, Fujia;Lu, Ying;Yuan, Bing;Tan, Zhi-Jie;Zhang, Xing-Hua;Dai, Liang published 《Twist-diameter coupling drives DNA twist changes with salt and temperature》 in 2022. The article was appeared in 《Science Advances》. They have made some progress in their research.Synthetic Route of ClRb The article mentions the following:
DNA deformations upon environmental changes, e.g., salt and temperature, play crucial roles in many biol. processes and material applications. Here, our magnetic tweezers experiments observed that the increase in NaCl, KCl, or RbCl concentration leads to substantial DNA overwinding. Our simulations and theor. calculation quant. explain the salt-induced twist change through the mechanism: More salt enhances the screening of interstrand electrostatic repulsion and hence reduces DNA diameter, which is transduced to twist increase through twist-diameter coupling. We determined that the coupling constant is 4.5 ± 0.8 kBT/(degrees·nm) for one base pair. The coupling comes from the restraint of the contour length of DNA backbone. On the basis of this coupling constant and diameter-dependent DNA conformational entropy, we predict the temperature dependence of DNA twist Δωbp/ΔT ≈ -0.01 degree/°C, which agrees with our and previous exptl. results. Our anal. suggests that twist-diameter coupling is a common driving force for salt- and temperature-induced DNA twist changes.Rubidiumchloride (cas: 7791-11-9) were involved in the experimental procedure.
Rubidium chloride(cas: 7791-11-9) is the mostly used rubidium compound, and finds use in various fields ranging from electrochemistry to molecular biology.Synthetic Route of ClRb It is an efficient non-invasive biomarker; increases dopamine and norepinephrine levels, and useful for anergic and apathetic depressives.
Reference:
Chloride – Wikipedia,
Chlorides – an overview | ScienceDirect Topics